Bile-Salt-Hydrolases from the Probiotic Strain Lactobacillus johnsonii La1 Mediate Anti-giardial Activity in Vitro and in Vivo

Frontiers in Microbiology
Thibault AllainIsabelle Florent

Abstract

Giardia duodenalis (syn. G. lamblia, G. intestinalis) is the protozoan parasite responsible for giardiasis, the most common and widely spread intestinal parasitic disease worldwide, affecting both humans and animals. After cysts ingestion (through either contaminated food or water), Giardia excysts in the upper intestinal tract to release replicating trophozoites that are responsible for the production of symptoms. In the gut, Giardia cohabits with the host's microbiota, and several studies have revealed the importance of this gut ecosystem and/or some probiotic bacteria in providing protection against G. duodenalis infection through mechanisms that remain incompletely understood. Recent findings suggest that Bile-Salt-Hydrolase (BSH)-like activities from the probiotic strain of Lactobacillus johnsonii La1 may contribute to the anti-giardial activity displayed by this strain. Here, we cloned and expressed each of the three bsh genes present in the L. johnsonii La1 genome to study their enzymatic and biological properties. While BSH47 and BSH56 were expressed as recombinant active enzymes, no significant enzymatic activity was detected with BSH12. In vitro assays allowed determining the substrate specificities of both BSH47 and ...Continue Reading

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Citations

May 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lifeng LiangGengyun Zhang
Sep 21, 2018·Frontiers in Cellular and Infection Microbiology·Ivet A YordanovaSusanne Hartmann
Jan 30, 2021·Frontiers in Microbiology·Elena FeketeAndre G Buret
Apr 28, 2021·Probiotics and Antimicrobial Proteins·K S YohaC Anandharamakrishnan
Jun 3, 2021·Microorganisms·Mélanie BourginMoez Rhimi

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Methods Mentioned

BETA
PCR
Electrophoresis
FCS
scanning
Scanning Electron

Software Mentioned

ClustalO
Pymol PyMOL Molecular Graphics System
GraphPad
pStaby
MEGA5
TASSER
BLASTP
Prism

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