Biliary anionic peptide fraction and apoA-I regulate intestinal cholesterol uptake

Biochemical and Biophysical Research Communications
Dominique Jourdheuil-RahmaniDenis Lairon

Abstract

Evidence is now in favor of protein-facilitated mechanisms for the intestinal cholesterol absorption. Here we report that the unesterified cholesterol uptake by rat jejunal brush border membrane vesicles (BBMVs) is efficient, saturable, and protein-mediated. The human apolipoproteins biliary anionic peptide factor (APF) and A-I (apoA-I) up-regulate micellar cholesterol uptake in a dose-dependent manner, but for all tested concentrations (0.1-20 microM), the lipid-free APF was more efficient than apoA-I. This uptake stimulation was suppressed after addition of Pabs directed to the external lipid-binding domain of the CLA-1/SR-BI and reduced by Pabs directed to the external loop of CD36. Thus, CLA-1/SR-BI and to a lesser extent CD36 are involved in the regulation of intestinal cholesterol uptake. APF, the main protein bound to biliary lipids, is likely one of their physiological effectors. As APF is an unesterified cholesterol carrier, it could facilitate the intestinal absorption of biliary cholesterol.

References

Feb 1, 1988·Proceedings of the Society for Experimental Biology and Medicine·M MartigneJ Hauton
May 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H LafontA Veis
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·E SehayekJ L Breslow
Jul 21, 2000·Biochimica Et Biophysica Acta·M HernandezY S Chao
Sep 11, 2001·Biochemical and Biophysical Research Communications·M J van WijlandA K Groen
Sep 19, 2001·The Journal of Clinical Investigation·M FebbraioR L Silverstein
Sep 19, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M V LoboR Bragado

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Citations

Dec 29, 2005·The Journal of Biological Chemistry·Emmanuelle ReboulPatrick Borel
Jul 3, 2003·Journal of Lipid Research·Joelle KasboIbrahim M Yousef
Nov 18, 2003·Journal of Lipid Research·Lei CaiDeneys R van der Westhuyzen
Oct 28, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Linda S ZhangPatrick Tso
Oct 3, 2003·Biochemical and Biophysical Research Communications·Barbara PlayDominique Jourdheuil-Rahmani
Aug 11, 2005·Metabolism: Clinical and Experimental·Nicole DomingoFrançoise Chanussot
Sep 16, 2005·Journal of Medicinal Chemistry·Lisbet KvaernøErick M Carreira

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