Biliary D-glucaric acid: its quantitation and preventive role in gallstone formation

Scandinavian Journal of Gastroenterology
Y D Ho, K J Ho

Abstract

A method for quantitation of D-glucaric acid in bile has been developed involving extraction with tetrahexylammonium chloride, boiling for 40-60 min, and determination of the percentage inhibition of beta-glucuronidase activity at 56 degrees C and pH 4. D-glucaric acid, bilirubin, bile acid, and protein were determined in 106 human gallbladder biles obtained at autopsy, including 20 with gallstones. The mean D-glucaric acid content was 1125 +/- 159 microM (mean +/- SE). Biliary beta-glucuronidase activity was not affected by D-glucaric acid because of 1) no difference in biliary D-glucaric acid content, either absolute or corrected for per unit of bilirubin, bile acid, or protein, between those with and those without gallstones; 2) no negative correlation between D-glucaric acid content and beta-glucuronidase activity in the bile; and 3) minimal conversion of D-glucaric acid to D-glucaro-1,4-lactone at the usual pH of bile. We conclude that biliary D-glucaric acid plays no role in the prevention of gallstone formation.

Citations

Feb 1, 1979·Prostaglandins·W BartmannB Schölkens
Apr 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A Macfadyen, K J Ho
Nov 2, 1973·Biochimica Et Biophysica Acta·W SakamotoE Sakakibara
Jan 1, 1982·Acta Pathologica Japonica·K TobeT Kobayashi
Sep 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J T LaMontJ R Moore
Mar 14, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·A ColombiV Foà
Jul 1, 1981·Proceedings of the Society for Experimental Biology and Medicine·K J Ho, L H Ho
Nov 1, 1980·European Journal of Clinical Pharmacology·K FiedlerH Cramer
Jan 1, 1960·Methods of Biochemical Analysis·P TALALAY
May 3, 1963·Biochemical and Biophysical Research Communications·H J BLUMENTHAL, D C FISH
Feb 1, 1963·Biochemical Pharmacology·A KIYOMOTOT MORITA

Related Concepts

Tetrahexylammonium chloride
Bile Acid Measurement
Study, Methodological
Autopsy
Bilirubin
Gallbladder (Mmhcc)
Gallstone Formation
Serum Bilirubin Measurement
Biliary Calculi
Blood Bilirubin Measurement

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