Biliary intervention augments chemotactic reaction and aggravates cholestatic liver injury in rats

The Journal of Surgical Research
Jiin-Haur ChuangTsu-Kung Lin

Abstract

Intervention of the biliary system is frequently done in patients with obstructive jaundice and is associated with significant morbidity and mortality. The pathogenesis is unknown. A rat model of bile duct ligation (BDL) for 2 weeks was established in which biliary intervention was feasible by injection of normal saline through an indwelling catheter in the bile ducts. Plasma levels of C-C chemokine MCP-1 and C-X-C chemokine MIP-2 were measured by using ELISA. Blood monocytes, Kupffer cells, and neutrophils in the liver were characterized with antibodies to ED1, ED2, and myeloperoxidase (MPO). Lipid peroxidation was measured by malondialdehyde contents and apoptosis by TUNEL stain of the liver. Biliary intervention resulted in an increase of plasma MCP-1 and MIP-2 proteins by 1 h, which declined to normal level by 3 h in both sham and BDL rats. The levels in BDL rats were significantly higher than in sham at most points. There was a transient increase of ED1- and ED2-positive cells and MPO-staining cells in sham rat liver by 1 h after intervention. ED2-positive cells increased significantly by 1 h, while ED1- and MPO-positive cells decreased, yet insignificantly after intervention in BDL rats. The cell counts in BDL were consta...Continue Reading

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Citations

Dec 24, 2005·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·P K BaierU T Hopt
Jan 31, 2014·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Jen-Bin WangTaiwai Chin
Dec 18, 2007·American Journal of Surgery·Bulent KilicogluMehmet Ali Akkus
May 27, 2005·The Journal of Surgical Research·Brygida BeckJashovam Shani
Jan 9, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Katsutaka WatanabeMasato Nagino
Jun 20, 2014·Experimental and Therapeutic Medicine·You-Xing ZhouXiaohong Liu

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