Biliary tract external drainage improves inflammatory mediators and pathomorphology of the intestine, liver, and lung in septic rats

The Journal of Trauma and Acute Care Surgery
Yong-Jun LiuXiang-Dong Guan

Abstract

To investigate the effect of biliary tract external drainage (BTED) on inflammatory mediators and pathomorphism of intestine, liver, and lung in septic rats. 48 SD rats (n = 8 per group) were randomized into six groups: control, sepsis, sepsis plus BTED, normal bile (obtained from eight healthy rats), and septic bile infusion for 6 hours respectively to test the effects of BTED bile infusion on cytokines' expression and tissue injury in the intestine, liver, and lung of septic/normal rats. Co-cultivation of intestinal epithelial cells (IEC-6) with bile for 12 hours was performed to evaluate the potential cytotoxicity of septic bile. Survival rate for sepsis plus BTED rats was detected compared with sepsis without BTED group (n = 20 per group) at 24, 48, and 72 hours, respectively. BTED for 6 hours significantly reduced the mRNA expression levels of tumor necrosis factor alpha (TNF-α) and IL-1β (all p < 0.05 vs. sepsis group), whereas mRNA expression of TNF-α and IL-1β in the intestine was increased after 6 hours' septic bile infusion compared with normal bile infusion group (all p < 0.05). TNF-α concentration in septic bile was significantly higher than that in the control group (p < 0.001). Tissue injury was significantly atte...Continue Reading

References

Feb 1, 1988·American Journal of Surgery·A J SoriG W Machiedo
May 1, 1985·Gut·B M MullockR H Hinton
Jan 25, 2000·The Journal of Surgical Research·S TowfighC Chandler
May 12, 2000·Immunological Reviews·P A Knolle, G Gerken
Jul 19, 2000·Infection and Immunity·G D JacksonW A Sewell
Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Jan 17, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Melissa D HalpernBohuslav Dvorak
Aug 18, 2004·Critical Care : the Official Journal of the Critical Care Forum·Eliézer SilvaUNKNOWN Brazilian Sepsis Epidemiological Study
Mar 21, 2007·World Journal of Surgery·Evangelos MessarisManousos M Konstadoulakis
Apr 28, 2007·Surgery·Cynthia L Leaphart, J J Tepas
Jul 5, 2007·Current Opinion in Infectious Diseases·Ismail Cinel, R Phillip Dellinger
Jan 17, 2008·The Journal of Toxicological Sciences·Kiyoshi NagataShuhei Sakaguchi
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Dec 17, 2009·Cell Death and Differentiation·T Vanden BergheP Vandenabeele
Mar 20, 2010·World Journal of Gastroenterology : WJG·H Joe WangM Katherine Jung
Mar 20, 2010·World Journal of Gastroenterology : WJG·Gyongyi Szabo, Shashi Bala
Jan 1, 2010·Digestive Diseases·Gyongyi SzaboArijeet Gattu
Sep 14, 2012·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yasuhiro Miyake, Kazuhide Yamamoto
Aug 24, 2013·BioMed Research International·Giuseppe ServilloBruno Memoli
Aug 30, 2013·The New England Journal of Medicine·Derek C Angus, Tom van der Poll
Sep 13, 2013·Critical Care : the Official Journal of the Critical Care Forum·Alexander KochKai Zacharowski
Dec 3, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Anne Marie BakkeÅshild Krogdahl
May 3, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Markus Huber-LangTom E Mollnes
Aug 28, 2014·Seminars in Cell & Developmental Biology·Sandrine Jouan-LanhouetPeter Vandenabeele
Nov 21, 2014·Cell Death & Disease·M AzizP Wang
Dec 3, 2014·Mayo Clinic Proceedings·Kelly A Cawcutt, Steve G Peters

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