Bilirubin accumulation and Cyp mRNA expression in selected brain regions of jaundiced Gunn rat pups.

Pediatric Research
Silvia GazzinClaudio Tiribelli

Abstract

Few data exist on regional brain bilirubin content in the neonatal period when acute bilirubin-induced neurologic damage (BIND) may occur, and no information is available on regional brain expression of cytochrome P450 monooxygenases (Cyps) that oxidize bilirubin. Bilirubin content was analyzed by high-performance liquid chromatography and Cyp1a1, 1a2, and 2a3 mRNA expression was analyzed by quantitative PCR (qPCR) in cortex (Cx), cerebellum (Cll), superior colliculi (SC), and inferior colliculi (IC) of 17-d-old hyperbilirubinemic (jj) Gunn rat pups before and after administration of sulphadimethoxine to acutely displace bilirubin from plasma albumin. There was no difference in bilirubin content among brain regions in untreated rats. After intraperitoneal sulphadimethoxine, bilirubin content peaked at fourfold in Cx and SC at 1 h; but at 11- to 13-fold in Cll and IC at 24 h; returning to control levels at 72 h. The Cyp mRNA peaked at 30-70 times control at 1 h in Cx and SC, but at 3-9 times control at 24 h in Cll and IC. The close relationship in distinct brain regions between the extent of bilirubin accumulation and induction of mRNA of Cyps suggests Cyps may have a role in protecting selected brain areas from bilirubin neurot...Continue Reading

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Citations

Nov 22, 2013·The New England Journal of Medicine·Jon F Watchko, Claudio Tiribelli
May 1, 2013·Toxicology and Applied Pharmacology·Sangsoo Daniel KimGordon M Kirby
Nov 6, 2014·Archives of Toxicology·Sabrina E GambaroSilvia Gazzin
Aug 23, 2016·Oxidative Medicine and Cellular Longevity·Jaroslav ZelenkaLibor Vítek
Aug 16, 2016·Trends in Molecular Medicine·Silvia GazzinClaudio Tiribelli
Aug 23, 2016·Neurobiology of Disease·Fernanda MarquesJoana Almeida Palha
Dec 23, 2016·Liver International : Official Journal of the International Association for the Study of the Liver·Silvia GazzinClaudio Tiribelli
Sep 1, 2016·Archives of Toxicology·Mohammed QaisiyaCristina Bellarosa
Feb 6, 2017·Cell Transplantation·Fu-Chen YangJohn A Stanford
Oct 11, 2019·Pediatric Research·Sean M Riordan, Steven M Shapiro
Sep 8, 2019·Pediatric Research·Giulia Bortolussi, Andrés F Muro
Sep 14, 2018·Scientific Reports·Eleonora VianelloSilvia Gazzin
Nov 5, 2019·Frontiers in Pharmacology·Xinyi LiShankai Yin

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