PMID: 9545555Apr 18, 1998Paper

Bilirubin as an anti precipitant against copper mediated denaturation of bovine serum albumin: formation of copper-bilirubin complex

Biochimica Et Biophysica Acta
S AdhikariC Gopinathan

Abstract

BR forms a beautiful 1:1 greenish complex with Cu2+, having a characteristic absorption maximum at 343 nm. To our knowledge, this is the first metal complex of bilirubin in aqueous solution so far to be reported. It has also been shown that bilirubin (BR) at a concentration more than protein binding capacity has a definite role as an anti precipitant of bovine serum albumin by excess copper. At a concentration of 1 x 10(-3) moll-1 of the Cu-BR complex, the colour becomes greenish black. The rate of formation of this Cu-BR complex when BR extracts copper from copper-albumin complex as obtained in our experiment is 34.981 mol-1 s-1. The Cu-BR complex is stable at a pH ranging from 3.5 to 13.2, and also can scavenge radicals like CCl3OO., eaq- and OH.. Addition of excess copper sulfate to the solution of this complex causes a greenish black precipitate which can be re dissolved in HCl but insoluble in 99% ethanol. The complex does not give positive test in Gmelin reaction for bile pigments. The fluorescence spectrum of the complex in solution exhibits a peak at 450 nm when excited at 343 nm. The precipitated complex is insoluble in a number of solvents like ether, xylene, benzene, acetone, chloroform-methanol mixture and DMSO. The...Continue Reading

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Citations

Apr 3, 2001·Phytochemistry·M DurantiF Sessa
Aug 6, 1999·Biochimica Et Biophysica Acta·S F AsadS M Hadi
Aug 22, 2013·Metallomics : Integrated Biometal Science·Vincent BalterFrancis Albarède
Jul 11, 2016·Journal of Pharmaceutical and Biomedical Analysis·Ivan D PavićevićLjuba M Mandić
Dec 16, 2016·Scientific Reports·Srestha BasuArun Chattopadhyay
Apr 12, 2017·Journal of Clinical Laboratory Analysis·Alain NicolayHenri Portugal

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