Bilirubin exhibits a novel anti-cancer effect on human adenocarcinoma

Biochemical and Biophysical Research Communications
Parijatha RaoShiro Sasaguri

Abstract

Anti-oxidants are essential for intracellular free radical scavenging, as free radicals are one of the causes for tumorigenesis. Our objective was to use bilirubin and investigate its action on human carcinoma cell lines. Bilirubin manifested as a prooxidant showing its cytopathic effect on TMK-1, showing growth inhibition close to 50%. Cell cycle analysis showed an arrest at G0/G1. Flow cytometry investigations with Red CC-1 showed an increase by more than 2 times suggesting a prooxidative role of bilirubin. To check the effect of radicals on DNA, a Comet Assay displayed a typical comet's tail with bilirubin treated slides, only. Further, staining with DAPI showed apoptotic action of bilirubin. Decreased mitochondrial function by bilirubin was observed with Mitotracker Green FM staining. These unexpected data have led us to conclude that bilirubin has anti-cancer activity as a prooxidant and may have a more vital role in the human body than realized.

References

Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Feb 7, 2003·Mini Reviews in Medicinal Chemistry·Ross P McGearyIstvan Toth
Apr 22, 2003·The Tohoku Journal of Experimental Medicine·Takashi OhruiHidetada Sasaki
Jul 26, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Andrew D PresleyEdgar A Arriaga
Feb 21, 2004·Toxicological Sciences : an Official Journal of the Society of Toxicology·Anabela P RoloKendall B Wallace
Jul 29, 2004·Cancer Causes & Control : CCC·Paloma CejasManuel González-Barón

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Citations

Apr 18, 2008·Anti-cancer Drugs·Karri BarabasChristopher Adin
Nov 8, 2012·Biological & Pharmaceutical Bulletin·Sa Rang OhJi Wook Jung
May 1, 2012·Alimentary Pharmacology & Therapeutics·M HjelkremS A Harrison
Dec 19, 2012·Experimental Cell Research·Christine MölzerKarl-Heinz Wagner
Aug 4, 2016·Angewandte Chemie·Yonghyun LeeSangyong Jon
Aug 3, 2011·Journal of Leukocyte Biology·Nazir M Khan, T B Poduval
Jun 25, 2020·Journal of Immunoassay & Immunochemistry·Syed Saqib AliFahim Halim Khan
Feb 13, 2018·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Hongchuang ZhangXiaomeng Song
Aug 29, 2013·Cancer Prevention Research·Marlies WallnerKarl-Heinz Wagner
Dec 8, 2020·American Journal of Physiology. Endocrinology and Metabolism·Justin F CreedenTerry D Hinds
Jul 3, 2007·Mutation Research·A C BulmerK-H Wagner
Aug 17, 2021·Oxidative Medicine and Cellular Longevity·Kamal Fatima ZahraDaniel Timofte

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