Bilirubin, model membranes and serum albumin interaction: The influence of fatty acids

Biochimica Et Biophysica Acta
Pavlína Novotná, Marie Urbanová

Abstract

Electronic circular dichroism (ECD), absorption and fluorescence spectroscopy were used to study the enantioselective interactions which involved bilirubin (BR), liposomes, human serum albumin of two different purities, pure (HSA) and non-purified of fatty acids (FA-HSA), and individual fatty acids. The application of the ECD technique to such a complex problem provided a new perspective on the BR binding to liposomes. Our results demonstrated that in the presence of pure HSA, BR preferred to bind to the protein over the liposomes. However, in the presence of FA-HSA, BR significantly bound to the liposomes composed either of DMPC or of sphingomyelin and bound only moderately to the primary and secondary binding sites of FA-HSA even at high BR concentrations. For the DMPC liposomes, even a change of BR conformation upon binding to the primary binding site was observed. The individual saturated fatty acids influenced the BR binding to HSA and liposomes in a similar way as fatty acids from FA-HSA. The unsaturated fatty acids interacted with BR alone and prevented it from interacting with either 99-HSA or the liposomes. In the presence of arachidonic acid, BR interacted enantioselectively with the liposomes and only moderately with...Continue Reading

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Dec 21, 2013·Biochimica Et Biophysica Acta·Pavlína NovotnáMarie Urbanová

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Citations

Jun 15, 2015·Biophysical Chemistry·Pavlína NovotnáMarie Urbanová
Dec 30, 2017·Free Radical Research·Mark A RosenfeldAnna V Bychkova
May 4, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ilia A Dereven'kovOskar I Koifman
Sep 28, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Song-Di NiHong-Ming Ding

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