Bilirubin oxidase from Magnaporthe oryzae: an attractive new enzyme for biotechnological applications.

Applied Microbiology and Biotechnology
Fabien DurandNicolas Mano

Abstract

A novel bilirubin oxidase (BOD), from the rice blast fungus Magnaporthe oryzae, has been identified and isolated. The 64-kDa protein containing four coppers was successfully overexpressed in Pichia pastoris and purified to homogeneity in one step. Protein yield is more than 100 mg for 2 L culture, twice that of Myrothecium verrucaria. The k(cat)/K(m) ratio for conjugated bilirubin (1,513 mM⁻¹ s⁻¹) is higher than that obtained for the BOD from M. verrucaria expressed in native fungus (980 mM⁻¹ s⁻¹), with the lowest K(m) measured for any BOD highly desirable for detection of bilirubin in medical samples. In addition, this protein exhibits a half-life for deactivation >300 min at 37 °C, high stability at pH 7, and high tolerance towards urea, making it an ideal candidate for the elaboration of biofuel cells, powering implantable medical devices. Finally, this new BOD is efficient in decolorizing textile dyes such as Remazol brilliant Blue R, making it useful for environmentally friendly industrial applications.

References

Nov 1, 1991·Applied Biochemistry and Biotechnology·J GuoG X Li
Aug 15, 1985·Archives of Biochemistry and Biophysics·K KawaharaA Nakahara
Dec 1, 1987·Clinical Biochemistry·A KosakaK Nakane
May 1, 1994·Journal of Biochemistry·J HiroseS Murao
Sep 23, 1997·Biochemical and Biophysical Research Communications·H J ZhangJ M Zhou
Apr 4, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·K KurosakaH Kondo
Nov 7, 1999·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·V N ZaitsevP F Lindley
Jan 21, 2000·FEMS Microbiology Reviews·J L Cereghino, J M Cregg
Aug 14, 2001·Bioresource Technology·Y Fu, T Viraraghavan
May 30, 2002·Journal of the American Chemical Society·Nicolas ManoAdam Heller
Jul 20, 2002·Phytochemistry·Alfred M Mayer, Richard C Staples
Oct 31, 2002·Angewandte Chemie·Edward I. SolomonAmy E. Palmer
Apr 1, 1960·Archives of Biochemistry and Biophysics·G FELSENFELD
Dec 11, 2003·Journal of the American Chemical Society·Nicolas ManoAdam Heller
Jan 27, 2005·Chemical Reviews·Scott Calabrese BartonPlamen Atanassov
Jun 1, 2005·Bioelectrochemistry·Chan KangAdam Heller
Jan 5, 2006·Applied and Environmental Microbiology·Shin-ichi SakasegawaToshihisa Ohshima
Feb 14, 2006·FEMS Microbiology Reviews·Petr Baldrian
Oct 25, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Paulo DurãoLígia O Martins
Mar 12, 2008·Applied Microbiology and Biotechnology·Katja KoschorreckVlada B Urlacher
Apr 23, 2008·Current Protocols in Protein Science·D R Higgins
Aug 30, 2008·Annals of Clinical Biochemistry·Jean M Kirk
Oct 22, 2008·Journal of Biotechnology·Luciana PereiraLígia O Martins
Apr 11, 2009·Biotechnology Letters·Kristiina HildénTaina Lundell
Oct 22, 2009·Cellular and Molecular Life Sciences : CMLS·Paola GiardinaGiovanni Sannia
Nov 17, 2009·Journal of Bioscience and Bioengineering·Youxun LiuXiaoyu Zhang
Oct 27, 2010·Nature Communications·Feng GaoNicolas Mano
Jan 27, 2011·BMC Biotechnology·Renate ReissLinda Thöny-Meyer
Oct 7, 2011·Chemical Communications : Chem Comm·Saravanan RengarajDónal Leech
Nov 23, 2011·Biosensors & Bioelectronics·Xiaoju WangSergey Shleev

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Citations

Aug 11, 2012·Applied Microbiology and Biotechnology·Nicolas Mano
Aug 6, 2013·Biosensors & Bioelectronics·Nicolas Mano, Lise Edembe
Apr 24, 2016·Biosensors & Bioelectronics·Marine CadetNicolas Mano
Dec 12, 2012·Protein Expression and Purification·Fabien DurandNicolas Mano
Dec 21, 2016·ACS Applied Materials & Interfaces·Deepak JainPhilippe Barthélémy
Apr 27, 2017·Lab on a Chip·Andrew G MarkPeer Fischer
Feb 1, 2018·Biosensors·Carla Gonzalez-Solino, Mirella Di Lorenzo
Feb 13, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Marine CadetNicolas Mano
Oct 5, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Chiara Di BariAntonio L De Lacey
Jul 21, 2017·Biotechnology Letters·Vinita HoodaVikas Hooda
Aug 15, 2018·Nature Communications·Emmanuel SuranitiAlexander Kuhn
Sep 21, 2017·Chemical Reviews·Nicolas Mano, Anne de Poulpiquet
Oct 12, 2021·Journal of the American Chemical Society·Alina SekretarevaEdward I Solomon
Oct 16, 2021·Nature Chemistry·Serena ArnaboldiAlexander Kuhn

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