Biliverdin Reductase A (BVRA) Knockout in Adipocytes Induces Hypertrophy and Reduces Mitochondria in White Fat of Obese Mice.

Biomolecules
David E StecTerry D Hinds

Abstract

Biliverdin reductase (BVR) is an enzymatic and signaling protein that has multifaceted roles in physiological systems. Despite the wealth of knowledge about BVR, no data exist regarding its actions in adipocytes. Here, we generated an adipose-specific deletion of biliverdin reductase-A (BVRA) (BlvraFatKO) in mice to determine the function of BVRA in adipocytes and how it may impact adipose tissue expansion. The BlvraFatKO and littermate control (BlvraFlox) mice were placed on a high-fat diet (HFD) for 12 weeks. Body weights were measured weekly and body composition, fasting blood glucose and insulin levels were quantitated at the end of the 12 weeks. The data showed that the percent body fat and body weights did not differ between the groups; however, BlvraFatKO mice had significantly higher visceral fat as compared to the BlvraFlox. The loss of adipocyte BVRA decreased the mitochondrial number in white adipose tissue (WAT), and increased inflammation and adipocyte size, but this was not observed in brown adipose tissue (BAT). There were genes significantly reduced in WAT that induce the browning effect such as Ppara and Adrb3, indicating that BVRA improves mitochondria function and beige-type white adipocytes. The BlvraFatKO m...Continue Reading

References

Aug 1, 1989·Cryobiology·B J FullerW De Loecker
Sep 5, 2003·American Journal of Physiology. Endocrinology and Metabolism·J G GrannemanL A Schwamb
Sep 18, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Shelby C YoungDavid E Stec
Jul 28, 2010·Molecular Endocrinology·Terry D HindsEdwin R Sanchez
Nov 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Barbara WegielLeo E Otterbein
Jan 3, 2012·Nutrition·Maryline DrouetBenjamin Bertin
Mar 23, 2012·Frontiers in Pharmacology·Barbara Wegiel, Leo E Otterbein
May 29, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Julien AuffretNadine Binart
Jan 11, 2013·Physiological Reviews·André Tchernof, Jean-Pierre Després
May 22, 2013·International Journal of Obesity : Journal of the International Association for the Study of Obesity·P A HosickD E Stec
Jun 7, 2013·Nutrients·Pavankumar Patel, Nicola Abate
Feb 1, 2014·Methods in Enzymology·Sebastian D ParleeOrmond A MacDougald
Feb 27, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peter E M GibbsMahin D Maines
Feb 27, 2014·Archives of Orthopaedic and Trauma Surgery·Philip J BelmontAndrew J Schoenfeld
Mar 13, 2014·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Richard Wa Mackenzie, Bradley T Elliott
May 13, 2014·The Journal of Biological Chemistry·Lance A StechschulteTerry D Hinds
Jun 17, 2014·Molecular Endocrinology·Lance A StechschulteEdwin R Sanchez
Jun 17, 2014·Molecular Endocrinology·Lance A StechschulteEdwin R Sanchez
Jan 13, 2015·Molecular and Cellular Endocrinology·Tamiris Lima RachidVanessa Souza-Mello
Mar 3, 2015·Trends in Endocrinology and Metabolism : TEM·Luke O'BrienTerry D Hinds
Sep 24, 2015·International Journal of Molecular Sciences·Zhizhi HuGang Xu
Oct 10, 2015·The Journal of Endocrinology·Amy Warner, Jens Mittag
Dec 25, 2015·Free Radical Biology & Medicine·Eugenio BaroneMarzia Perluigi
Apr 14, 2016·PloS One·David E StecTerry D Hinds
May 6, 2016·Frontiers in Endocrinology·Sung Sik ChoeJae Bum Kim
May 12, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tihomir MiralemMahin D Maines
Jun 25, 2016·International Journal of Molecular Sciences·Wan-Xia ZhangYou-E Yan
Jul 22, 2016·Endocrinology·Lance A StechschulteEdwin R Sanchez
Aug 23, 2016·Frontiers in Cell and Developmental Biology·Marco RaffaeleLuca Vanella
Sep 22, 2016·American Journal of Physiology. Endocrinology and Metabolism·Jordan GainesEdward O Bixler
Jan 18, 2017·American Journal of Physiology. Endocrinology and Metabolism·Terry D HindsDavid E Stec

❮ Previous
Next ❯

Citations

Dec 3, 2020·International Journal of Molecular Sciences·Valentina CeccarelliMaria Gisella Cavallo
Dec 18, 2020·Frontiers in Cellular Neuroscience·Peter SolárMarek Joukal
Dec 8, 2020·American Journal of Physiology. Endocrinology and Metabolism·Justin F CreedenTerry D Hinds
Dec 24, 2020·Nature Reviews. Molecular Cell Biology·Anthony J CovarrubiasEric Verdin
Apr 29, 2021·Antioxidants & Redox Signaling·Libor Vítek
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Darren M GordonTerry D Hinds
Jun 3, 2021·Antioxidants·David E Stec, Nader G Abraham
Jul 6, 2021·Free Radical Biology & Medicine·Cesare Mancuso

❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
PCR
Electrophoresis
Protein Assay
GTPase

Software Mentioned

LAS AF
Prism
GraphPad

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.