Biliverdin reduction by cyanobacterial phycocyanobilin:ferredoxin oxidoreductase (PcyA) proceeds via linear tetrapyrrole radical intermediates

Journal of the American Chemical Society
Shih-Long TuJ C Lagarias

Abstract

Cyanobacterial phycocyanobilin:ferredoxin oxidoreductase (PcyA) catalyzes the four electron reduction of biliverdin IXalpha (BV) to phycocyanobilin, a key step in the biosynthesis of the linear tetrapyrrole (bilin) prosthetic groups of cyanobacterial phytochromes and the light-harvesting phycobiliproteins. Using an anaerobic assay protocol, optically detected bilin-protein intermediates, produced during the PcyA catalytic cycle, were shown to correlate well with the appearance and decay of an isotropic g approximately 2 EPR signal measured at low temperature. Absorption spectral simulations of biliverdin XIIIalpha reduction support a mechanism involving direct electron transfers from ferredoxin to protonated bilin:PcyA complexes.

References

Apr 1, 2000·The Plant Journal : for Cell and Molecular Biology·K L WüthrichS Hörtensteiner
Jun 20, 2001·Biochemistry·M BollD J Lowe
Feb 14, 2002·Progress in Biophysics and Molecular Biology·H EklundP Nordlund
Jan 7, 2003·The Journal of Biological Chemistry·Nicole Frankenberg, J Clark Lagarias

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Citations

Jul 14, 2012·Plant Molecular Biology·Stefan Hörtensteiner
Aug 6, 2013·Archives of Biochemistry and Biophysics·Burak V KabasakalAndrew J Fisher
Nov 6, 2009·The Journal of Biological Chemistry·Yoshinori HagiwaraKeiichi Fukuyama
Sep 30, 2010·The Journal of Biological Chemistry·Jessica WiethausNicole Frankenberg-Dinkel
Jan 24, 2007·The Plant Cell·Adriana PruzinskáStefan Hörtensteiner
Dec 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yoshinori HagiwaraKeiichi Fukuyama
Jul 7, 2011·The Biochemical Journal·Andrea W U BuschEckhard Hofmann
Nov 6, 2010·The Biochemical Journal·Andrea W U BuschNicole Frankenberg-Dinkel
Oct 16, 2010·Biochemical and Biophysical Research Communications·Kei WadaKeiichi Fukuyama
Mar 8, 2016·Environmental Microbiology·Benjamin LedermannNicole Frankenberg-Dinkel
May 8, 2018·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Eri IijimaSeiji Mori
Oct 11, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Thorben Dammeyer, Nicole Frankenberg-Dinkel
May 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Christian Wiebeler, Igor Schapiro
Jul 30, 2008·The Journal of Biological Chemistry·Thorben DammeyerNicole Frankenberg-Dinkel
Jul 19, 2019·The FEBS Journal·Marco ArasNicole Frankenberg-Dinkel
Jul 29, 2008·The Journal of Biological Chemistry·Shih-Long TuLi-Wen Ku
Aug 7, 2014·The Journal of Biological Chemistry·Kristina E OverkampNicole Frankenberg-Dinkel
Oct 11, 2017·Journal of Experimental Botany·Benke KuaiStefan Hörtensteiner
Jul 22, 2006·The Journal of Biological Chemistry·Thorben Dammeyer, Nicole Frankenberg-Dinkel
Aug 2, 2019·The Journal of Biological Chemistry·Johannes A SommerkampEckhard Hofmann
Jan 12, 2017·The Journal of Biological Chemistry·Raphael GasperNicole Frankenberg-Dinkel
Apr 8, 2019·Current Opinion in Structural Biology·Masakazu SugishimaKeiichi Fukuyama
Aug 22, 2013·Biochemistry·Indra D SahuGary A Lorigan
Dec 21, 2010·Biochimica Et Biophysica Acta·Stefan Hörtensteiner, Bernhard Kräutler

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