BIM promoter directly targeted by EBNA3C in polycomb-mediated repression by EBV.

Nucleic Acids Research
Kostas PaschosMartin J Allday

Abstract

Detailed analyses of the chromatin around the BIM promoter has revealed that latent Epstein-Barr virus (EBV) triggers the recruitment of polycomb repressive complex 2 (PRC2) core subunits and the trimethylation of histone H3 lysine 27 (H3K27me3) at this locus. The recruitment is absolutely dependent on nuclear proteins EBNA3A and EBNA3C; what is more, epitope-tagged EBNA3C could be shown bound near the transcription start site (TSS). EBV induces no consistent changes in the steady-state expression of PRC2 components, but lentivirus delivery of shRNAs against PRC2 and PRC1 subunits disrupted EBV repression of BIM. The activation mark H3K4me3 is largely unaltered at this locus irrespective of H3K27me3 status, suggesting the establishment of a 'bivalent' chromatin domain. Consistent with the 'poised' nature of these domains, RNA polymerase II (Pol II) occupancy was not altered by EBV at the BIM TSS, but analysis of phospho-serine 5 on Pol II indicated that EBNA3A and EBNA3C together inhibit initiation of BIM transcripts. B cell lines carrying EBV encoding a conditional EBNA3C-oestrogen receptor-fusion revealed that this epigenetic repression of BIM was reversible, but took more than 3 weeks from when EBNA3C was inactivated.

References

Apr 1, 1997·The EMBO Journal·K Y Yankulov, D L Bentley
Mar 14, 1998·Journal of Virology·I Cludts, P J Farrell
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·H J DelecluseW Hammerschmidt
Oct 9, 1998·Genes & Development·C J Sherr
Mar 17, 1999·Nucleic Acids Research·R Wade-MartinsM R James
Apr 21, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G W Bornkamm, W Hammerschmidt
Apr 21, 2001·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D H Crawford
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Alexander EgleSuzanne Cory
Nov 3, 2004·Genes & Development·Giuseppina CarettiVittorio Sartorelli
Feb 19, 2005·Nature Reviews. Immunology·Andreas Strasser
Feb 22, 2005·BMC Molecular Biology·Xiaozhu ZhangAndrew J Sandford
Sep 20, 2005·Cancer Cell·Chi V DangTarja Juopperi
Dec 17, 2005·Nature·Emmanuelle ViréFrançois Fuks
Jan 31, 2006·Nature Genetics·Ilana KeshetItamar Simon
Apr 4, 2006·Nucleic Acids Research·Katrin NowakWerner Lutz
Dec 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Seiji MaruoKenzo Takada
Jan 4, 2007·Nature Genetics·Martin WidschwendterPeter W Laird
Apr 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael A CalderwoodEric Johannsen

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Citations

Dec 11, 2013·Infectious Agents and Cancer·Kenneth AlibekSamal Zhussupbekova
May 31, 2014·PLoS Pathogens·Charles TorgborDavid A Thorley-Lawson
Feb 15, 2014·The American Journal of Pathology·Natalie C Silmon de Monerri, Kami Kim
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sizun JiangBo Zhao
Apr 20, 2016·Frontiers in Microbiology·Shaoni BhattacharjeeAbhik Saha
Jan 11, 2013·Journal of Zhejiang University. Science. B·Qin FuZheng-rong Mao
Jan 24, 2015·Experimental & Molecular Medicine·Myung-Soo Kang, Elliott Kieff
May 21, 2016·Frontiers in Microbiology·Shuvomoy BanerjeeSubhash C Verma
Jul 28, 2016·Acta Biochimica Et Biophysica Sinica·Chao LiHongqi Liu
Oct 11, 2015·Current Opinion in Virology·Bettina Kempkes, Erle S Robertson
Nov 16, 2017·Viruses·Leah Fitzsimmons, Gemma L Kelly
Feb 2, 2018·Viruses·Makda GebreBenjamin E Gewurz
Feb 13, 2017·Oral Diseases·J T GuidryR S Scott
Aug 21, 2018·Annual Review of Pathology·Paul J Farrell
Nov 7, 2013·Nature Reviews. Microbiology·Paul M Lieberman
Apr 12, 2019·Journal of Virology·Abhik Saha, Erle S Robertson
Sep 4, 2019·Nature Reviews. Microbiology·Christian Münz
Jan 17, 2019·Nucleic Acids Research·Kostas PaschosMartin J Allday
Aug 16, 2016·Nature·Jonathan J KotzinJorge Henao-Mejia
May 4, 2017·Immunological Reviews·Kun-Po LiDavid A Hildeman

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Datasets Mentioned

BETA
GM12878

Methods Mentioned

BETA
ChIP
electrophoresis
PCR
immunoprecipitation
ChIPs
immunoprecipitations
ChIP-seq
co-immunoprecipitation

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