Bim siRNA decreases lymphocyte apoptosis and improves survival in sepsis

Shock
Steven J SchwulstR S Hotchkiss

Abstract

To assess the degree of lymphocyte apoptosis and survival in mice treated with small interfering RNA (siRNA) targeted to Bim, a proapoptotic molecule from the Bcl-2 family, within a clinically relevant model of sepsis. C57BL/6 mice were treated with a single dose of Bim siRNA complexed in cationic liposomes via tail vein injection. Approximately 24 h later, mice were subjected to either cecal ligation and puncture (CLP) or sham surgery. Animals were killed at 20 h postsurgery, and spleens were harvested for fluorescence-activated cell sorting analysis using terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling as a marker for apoptosis. A second cohort of mice was followed for survival for 7 days. The degree of lymphocyte apoptosis in Bim siRNA-treated mice was markedly decreased compared with controls. Fluorescent activated cell sorter analysis demonstrated 13.1% +/- 1.2% B-cell apoptosis and 11.5% +/- 1.5% T-cell apoptosis in control mice compared with 2.7% +/- 0.4% B-cell apoptosis and 3.9% +/- 0.3% T-cell apoptosis in Bim siRNA-treated mice after CLP (P < 0.001 and P < 0.01, respectively). This striking difference in lymphocyte apoptosis correlated with a significant survival advantage in Bim siRNA-tr...Continue Reading

References

Jan 11, 1995·JAMA : the Journal of the American Medical Association·M S Rangel-FraustoR P Wenzel
Jul 1, 1997·Critical Care Medicine·F ZeniC Natanson
Aug 14, 1999·Critical Care Medicine·R S HotchkissI E Karl
Dec 10, 1999·American Journal of Surgery·C D HeideckeB Holzmann
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·R S HotchkissI E Karl
Oct 18, 2000·The Journal of Experimental Medicine·S Roy, D W Nicholson
Mar 23, 2001·Nature Immunology·R S HotchkissI E Karl
May 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R S HotchkissI E Karl
Mar 21, 2002·Critical Care Medicine·Craig M CoopersmithRichard S Hotchkiss
Jan 10, 2003·The New England Journal of Medicine·Richard S Hotchkiss, Irene E Karl
Feb 1, 2003·Shock·Kenji SugimotoOlivier Pradier
Feb 13, 2003·Nature Medicine·Erwei SongJudy Lieberman
Mar 26, 2003·Journal of Molecular Biology·Dag R SørensenMouldy Sioud
Apr 18, 2003·The New England Journal of Medicine·Greg S MartinMarc Moss
Sep 10, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Akiko IwataRobert K Winn
Nov 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Marc PellegriniAndreas Strasser
Dec 4, 2003·Biochemical and Biophysical Research Communications·Mouldy Sioud, Dag R Sørensen
Jan 28, 2004·Cell·Nika N Danial, Stanley J Korsmeyer
Jun 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ursula BommhardtRichard S Hotchkiss
Jul 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joel G R WeaverAndrew D Badley
Oct 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Péter HamarJudy Lieberman
Nov 24, 2004·Nature Biotechnology·Jeremy D HeidelMark E Davis
Feb 19, 2005·Nature Reviews. Immunology·Andreas Strasser
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Richard S HotchkissIrene E Karl
Apr 9, 2005·Journal of Leukocyte Biology·Doreen E WescheAlfred Ayala
Jun 9, 2005·Blood·Doreen E Wesche-SoldatoAlfred Ayala
Dec 14, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Adam D JudgeIan MacLachlan
May 17, 2006·The Journal of Infectious Diseases·Thomas W GeisbertIan MacLachlan
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Steven J SchwulstRichard S Hotchkiss
Oct 14, 2006·Nature Reviews. Immunology·Richard S Hotchkiss, Donald W Nicholson
Dec 8, 2006·The New England Journal of Medicine·René Bernards
Jan 20, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tracey H WagnerJ Perren Cobb
Feb 10, 2007·Science·Tâm MignotDavid R Zusman
Feb 20, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katherine C ChangRichard S Hotchkiss
Apr 19, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Soo In KimMeehyein Kim
Apr 27, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·José I Martínez-FerrandisM-Eugenia Armengod

❮ Previous
Next ❯

Citations

Dec 17, 2009·Critical Care Medicine·Amy C FoxCraig M Coopersmith
Nov 27, 2008·Shock·Pavan BrahmamdamRichard S Hotchkiss
Mar 4, 2011·Shock·Shigeaki InoueRichard S Hotchkiss
Oct 18, 2008·Critical Care : the Official Journal of the Critical Care Forum·Stefan U WeberFrank Stüber
Jul 16, 2010·Critical Care : the Official Journal of the Critical Care Forum·Fanny Turrel-DavinFabienne Venet
Apr 1, 2009·Nanomedicine·Denis B Buxton
Jun 5, 2013·Chest·Alexandra L McCubbrey, Jeffrey L Curtis
Dec 9, 2014·The Journal of Surgical Research·Bernard J DuBrayChristopher D Anderson
Nov 30, 2011·Nanomedicine·Meir GoldsmithDan Peer
Nov 18, 2010·Journal of Pharmacological Sciences·Yuichi HattoriNaoyuki Matsuda
Apr 30, 2015·International Immunopharmacology·Joon-Sung KimSun-Mee Lee
Feb 24, 2009·Trends in Molecular Medicine·Ya-Ching HsiehIrshad H Chaudry
Jan 29, 2008·Journal of the American College of Surgeons·Steven J SchwulstRichard S Hotchkiss
Dec 15, 2010·Indian Pediatrics·M Kc Nair
Jan 30, 2014·The Journal of Biological Chemistry·Philippe M LeBlancMaya Saleh
Sep 29, 2020·Frontiers in Immunology·Zhenxing ChengGuozheng Wang
Jul 17, 2008·Shock·Stephen F Lowry
Apr 14, 2010·Shock·Kevin R KastenCharles C Caldwell
Apr 17, 2020·Advanced Drug Delivery Reviews·Nuphar VeigaDan Peer
Mar 28, 2013·Anaesthesia and Intensive Care·M HarjaiA B Pant
Aug 3, 2021·Frontiers in Pharmacology·Jian Chen, Haiming Wei
Feb 4, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Eizo WatanabeRichard S Hotchkiss
Apr 8, 2011·American Journal of Respiratory and Critical Care Medicine·Martina Victoria SchmidtAndreas von Knethen
Jul 8, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Amy C FoxCraig M Coopersmith

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis