Bimodal effects of luteinizing hormone and role of androgens in modifying superovulatory responses of rats to infusion with purified porcine follicle-stimulating hormone

Biology of Reproduction
D T ArmstrongY Chandrasekhar

Abstract

Prepubertal (28-30 days old) female rats were infused s.c. over a 60-h period with a purified porcine pituitary follicle-stimulating hormone (FSH) preparation having FSH specific activity 8.4 times that of NIH-FSH-S1 and luteinizing hormone (LH) specific activity less than 0.005 times that of NIH-LH-S1, based on radioreceptor assays. When the FSH infusion rate of this preparation was increased over the range of 0.5-2 units/day (mg NIH-FSH-S1 equivalent), an all-or-none response was observed, with the threshold dose for superovulation being between 1 and 2 units/day. Eleven of twelve rats receiving the 2 units/day dose ovulated a mean +/- SEM of 67 +/- 8 oocytes on the morning of the third day after the beginning of FSH infusion. Addition of human chorionic gonadotrophin (hCG), as a source of LH activity, to a subthreshold (1 U/day) FSH infusion rate resulted in 20% of rats ovulating at an hCG dosage of 50 mIU/day; increasing the hCG infusion to 200 mIU/day concomitant with the subthreshold FSH infusion rate increased ovulation rate to a mean of 69 +/- 8/rat, with 100% of rats ovulating. To determine the effect of varying both FSH infusion rates and LH:FSH ratios, FSH was infused at several rates, with hCG added to give varying ...Continue Reading

Citations

Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·A S McNeilly
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A Kanbour-ShakirT J Gill
Jan 1, 1991·Annals of the New York Academy of Sciences·D T ArmstrongF Khamsi
Feb 1, 1996·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·F Scheele, J Schoemaker
Apr 1, 1994·Journal of the Society for Gynecologic Investigation·G NemethF Naftolin

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