Bimodal Expression of the Salmonella Typhimurium spv Operon

Genetics
Ioannis PassarisAbram Aertsen

Abstract

The well-studied spv operon of Salmonellatyphimurium is important for causing full virulence in mice and both the regulation and function of the Spv proteins have been characterized extensively over the past several decades. Using quantitative single-cell fluorescence microscopy, we demonstrate the spv regulon to display a bimodal expression pattern that originates in the bimodal expression of the SpvR activator. The spv expression pattern is influenced by growth conditions and the specific Styphimurium strain used, but does not require Salmonella-specific virulence regulators. By monitoring real-time promoter kinetics, we reveal that SpvA has the ability to impart negative feedback on spvABCD expression without affecting spvR expression. Together, our data suggest that the SpvA protein counteracts the positive feedback loop imposed by SpvR, and could thus be responsible for dampening spvABCD expression and coordinating virulence protein production in time. The results presented here yield new insights in the intriguing regulation of the spv operon and adds this operon to the growing list of virulence factors exhibiting marked expression heterogeneity in Styphimurium.

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Citations

Oct 2, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sidi YangRui Huang
Mar 19, 2020·Micromachines·Phalguni Tewari KumarJeroen Lammertyn
Sep 27, 2019·Food Research International·Ines StaesAbram Aertsen
Apr 6, 2021·Frontiers in Cellular and Infection Microbiology·Sidi YangYuanyuan Li
Jul 3, 2021·Pathogens·Michał WójcickiEdyta Juszczuk-Kubiak

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