Bimodal viral vectors and in vivo imaging reveal the fate of human neural stem cells in experimental glioma model.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Khalid ShahRalph Weissleder

Abstract

Transplantation of genetically engineered cells into the CNS offers immense potential for the treatment of several neurological disorders. Monitoring expression levels of transgenes and following changes in cell function and distribution over time is critical in assessing therapeutic efficacy of such cells in vivo. We have engineered lentiviral vectors bearing fusions between different combinations of fluorescent and bioluminescent marker proteins and used bioluminescence imaging and intravital-scanning microscopy in real time to study the fate of human neural stem cells (hNSCs) at a cellular resolution in glioma-bearing brains in vivo. Using Renilla luciferase (Rluc)-DsRed2 or GFP-Rluc-expressing malignant human glioma model, transduced hNSCs were shown to migrate extensively toward gliomas, with hNSCs populating gliomas at 10 d after transplantation. Furthermore, transduced hNSCs survived longer in mice with gliomas than in normal brain, but did not modulate glioma progression in vivo. These studies demonstrate the utility of bimodal viral vectors and real-time imaging in evaluating fate of NSCs in diseased models and thus provide a platform for accelerating cell-based therapies for CNS disorders.

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Citations

Dec 18, 2012·Journal of Neuro-oncology·Shawn HingtgenKhalid Shah
Aug 30, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kaoru TamuraKhalid Shah
Mar 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Laura S SasportasKhalid Shah
Sep 24, 2010·Cold Spring Harbor Perspectives in Biology·John Condeelis, Ralph Weissleder
Nov 13, 2008·Molecular Cancer Therapeutics·Shawn HingtgenKhalid Shah
Dec 19, 2009·Regenerative Medicine·Atul GeraRaphael Guzman
May 31, 2013·Future Oncology·Boudewijn van der SandenDidier Wion
May 20, 2014·Journal of the National Cancer Institute·Matthias DuebgenKhalid Shah
Mar 25, 2014·Journal of Molecular Neuroscience : MN·Yebing ChenHuanxiang Zhang
Apr 1, 2008·The Lancet Oncology·Maarten F Corsten, Khalid Shah
Feb 8, 2013·Stem Cells·Jordi Martinez-QuintanillaKhalid Shah
Sep 1, 2015·Methods : a Companion to Methods in Enzymology·Juli Rodriguez BagóShawn D Hingtgen
Feb 14, 2015·CNS Neuroscience & Therapeutics·Yao-Hui TangGuo-Yuan Yang
Oct 28, 2014·Stem Cells·Daniel W StuckeyKhalid Shah
Jan 28, 2010·Neuropathology and Applied Neurobiology·J M A KuijlenW Helfrich
Apr 26, 2015·Brain : a Journal of Neurology·Tugba Bagci-OnderKhalid Shah
Jun 5, 2015·Frontiers in Molecular Neuroscience·Louise C Parr-BrownlieStephanie M Hughes
Jul 17, 2012·Cancer Treatment Reviews·Daniel BexellJohan Bengzon
Nov 2, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sung Hugh ChoiKhalid Shah
Jun 2, 2017·International Journal of Cancer. Journal International Du Cancer·Nusrat JahanHiroaki Wakimoto
Oct 28, 2017·Translational Stroke Research·Deepaneeta SarmahPallab Bhattacharya
Dec 9, 2008·Current Opinion in Organ Transplantation·Michel Modo
Jul 2, 2010·Molecular Medicine·Nora Sandu, Bernhard Schaller
Feb 19, 2016·Journal of Neurophysiology·Rachel J SizemoreLouise C Parr-Brownlie
Feb 13, 2016·Oncology Letters·Hiroki NambaTomohiro Yamasaki
Jul 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Wanlu DuKhalid Shah

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