Binding and aggregation of human gamma-globulin by cis-diamminedichloroplatinum(II) through disulfide bond

Biological & Pharmaceutical Bulletin
D ChenT Yotsuyanagi

Abstract

The incubation of gamma-globulin with cis-diamminedichloroplatinum (II) (cis-DDP) resulted in gradual formation of insoluble aggregates. Since the precipitates, composed of polymerized gamma-globulin and cis-DDP, were completely solubilized with urea, the reaction mixture containing precipitate was examined in terms of the binding of cis-DDP and the effect on disulfide (S-S) bonds in the gamma-globulin. When gamma-globulin was incubated with 30 molar excess cis-DDP at pH 7.4 and 37 degrees C, cis-DDP gradually bound to as much as 12 mol per mol of gamma-globulin in 14 d. Concurrently, about four disulfide bonds were cleaved without reaching a certain plateau. An sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the aggregated gamma-globulin induced by cis-DDP was significantly different from that of the heat-denaturated aggregate form or the reduced form by sulfitolysis. The aquated complexes of cis-DDP also produced an insoluble precipitate and affected the S-S bond to a greater extent than the parent drug.

Citations

Jul 17, 2012·Pharmacology & Therapeutics·Sandra M Sancho-MartínezFrancisco J López-Hernández
Apr 24, 2010·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Bernhard Michalke
Jan 18, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Raphael GorodetskyGerard Marx

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