PMID: 2111548May 1, 1990Paper

Binding and biological effects of tumor necrosis factor and gamma interferon in human pancreatic carcinoma cells

Pancreas
A B RaitanoMurray Korc

Abstract

The cytotoxic/cytostatic effects of recombinant human tumor necrosis factor alpha (rhTNF) and gamma interferon (rhIFN-gamma) were studied in five human pancreatic tumor cell lines. During a 48-h incubation, MIA PaCa-2 cells were most sensitive to rhTNF (56% cytotoxicity, 500 U/ml), T3M4 cells were most sensitive to rhIFN-gamma (54% cytostasis, 250 U/ml), and ASPC-1 and COLO 357 cells were most sensitive to the combination of rhTNF and rhIFN-gamma (56 and 55% cytotoxicity, respectively, 250 U/ml of each cytokine). The PANC-1 cells were relatively insensitive to either the individual or the combined effects of these cytokines. All five cell lines exhibited specific, high-affinity receptors for 125I-labeled rhTNF (480-8,610 sites/cell) and rhIFN-gamma (2,050-6,280 sites/cell). The MIA PaCa-2 cells, which were the most sensitive to the inhibitory effects of rhTNF, also possessed the largest number of 125I rhTNF receptors; all other cell lines had a relatively low number of binding sites and low sensitivity. In contrast, no direct correlation could be made between the number of IFN-gamma binding sites and inhibitory sensitivity in any of the cell lines. Incubation of COLO 357 cells at 37 degrees C with either 125I rhTNF or 125I rhIN...Continue Reading

Citations

May 9, 2000·Biochemical and Biophysical Research Communications·F OzawaM W Büchler
Dec 31, 2003·The Journal of Surgical Research·Robert Saeid FarivarEdward E Whang
Nov 24, 2001·Surgical Oncology·C J MageeJ P Neoptolemos
Jan 7, 2004·Biochemical and Biophysical Research Communications·Mark S DuxburyEdward E Whang
Aug 30, 2003·Surgery·Hiromichi ItoEdward E Whang
Aug 2, 2003·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Inken HilgendorfCharli Kruse
Oct 22, 2002·Expert Opinion on Biological Therapy·Øystein Bruserud, Nils Glenjen
Nov 19, 2003·Biochemical and Biophysical Research Communications·Mark S DuxburyEdward E Whang
Jun 15, 2004·Journal of the American College of Surgeons·Mark S DuxburyEdward E Whang
Apr 15, 2004·Biochemical and Biophysical Research Communications·Mark S DuxburyEdward E Whang
Jan 16, 2007·Biochemical and Biophysical Research Communications·Mark S Duxbury, Edward E Whang
Apr 13, 2000·International Journal of Cancer. Journal International Du Cancer·J KleeffM Korc
May 3, 1996·International Journal of Cancer. Journal International Du Cancer·M NaefM Korc
Dec 11, 1996·International Journal of Cancer. Journal International Du Cancer·M WagnerM Korc
Mar 9, 2005·World Journal of Gastroenterology : WJG·Elisabetta CeniAndrea Galli
Aug 7, 2018·Cell Biology International·Frank GieselerFanny Ender
Aug 1, 1996·Bioscience Reports·J B Dietrich
Sep 24, 2004·Annals of Surgery·Mark S DuxburyEdward E Whang
Apr 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mark S DuxburyEdward E Whang
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mark S DuxburyEdward E Whang
Mar 1, 1990·International Journal of Pancreatology : Official Journal of the International Association of Pancreatology·A B RaitanoM Korc

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.