Binding and functional properties of five extrinsic proteins in oxygen-evolving photosystem II from a marine centric diatom, Chaetoceros gracilis.

The Journal of Biological Chemistry
Ryo NagaoIsao Enami

Abstract

Oxygen-evolving photosystem II (PSII) isolated from a marine centric diatom, Chaetoceros gracilis, contains a novel extrinsic protein (Psb31) in addition to four red algal type extrinsic proteins of PsbO, PsbQ', PsbV, and PsbU. In this study, the five extrinsic proteins were purified from alkaline Tris extracts of the diatom PSII by anion and cation exchange chromatographic columns at different pH values. Reconstitution experiments in various combinations with the purified extrinsic proteins showed that PsbO, PsbQ', and Psb31 rebound directly to PSII in the absence of other extrinsic proteins, indicating that these extrinsic proteins have their own binding sites in PSII intrinsic proteins. On the other hand, PsbV and PsbU scarcely rebound to PSII alone, and their effective bindings required the presence of all of the other extrinsic proteins. Interestingly, PSII reconstituted with Psb31 alone considerably restored the oxygen evolving activity in the absence of PsbO, indicating that Psb31 serves as a substitute in part for PsbO in supporting oxygen evolution. A significant difference found between PSIIs reconstituted with Psb31 and with PsbO is that the oxygen evolving activity of the former is scarcely stimulated by Cl(-) and C...Continue Reading

References

Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Nov 12, 1996·Biochimica Et Biophysica Acta·A Seidler
Jan 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nobuo Kamiya, Jian-Ren Shen
Oct 2, 2003·European Journal of Biochemistry·Hisataka OhtaIsao Enami
Feb 7, 2004·Science·Kristina N FerreiraSo Iwata
Jan 4, 2007·Photosynthesis Research·Johnna L RooseHimadri B Pakrasi
Feb 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johnna L RooseHimadri B Pakrasi
Aug 22, 2008·Photosynthesis Research·Isao EnamiJian-Ren Shen
Feb 17, 2009·Nature Structural & Molecular Biology·Albert GuskovWolfram Saenger

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Citations

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Aug 2, 2011·Biochimica Et Biophysica Acta·Terry M BrickerJulian J Eaton-Rye
Sep 13, 2011·Biochimica Et Biophysica Acta·Lan-Xin ShiWolfgang P Schröder
Apr 8, 2015·Bioscience, Biotechnology, and Biochemistry·Kentaro Ifuku
Feb 1, 2012·Biochimica Et Biophysica Acta·Mercedes RoncelJosé M Ortega
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