PMID: 2427600Feb 1, 1986Paper

Binding and internalization of low-density lipoproteins in SCC25 cells and SV40 transformed keratinocytes. A morphologic study

The Journal of Investigative Dermatology
B J VermeerM Ponec

Abstract

Binding of low-density lipoproteins (LDL) to the plasma membrane and internalization of low-density lipoprotein receptor complexes were investigated in an epithelial tumor cell derived from the tongue (SCC25) and in SV40-transformed keratinocytes (SVK14 cells). For light microscopic studies an immunofluorescence technique with antiapoprotein B as well as conjugation procedure by which a fluorochrome 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanide (DIL) was conjugated with LDL (LDL-DIL) was used. Binding of LDL to the plasma membrane at 4 degrees C was observed in most SCC25 cells but not in SVK14 cells. The internalization of LDL-DIL was absent in SVK14 cells and was excessive in SCC25 cells. In SCC25 cells, internalization of the LDL-DIL particles was heterogeneously distributed over various cells. When a pulse-chase experiment was performed with LDL-DIL, less LDL was internalized into the SCC25 cells in comparison with a continuous label experiment. For the ultrastructural studies LDL conjugated with colloidal gold was used. In the binding experiments at 4 degrees C most LDL-gold particles were attached to the plasma membrane outside coated pits. During internalization experiments with LDL-gold particles it was observ...Continue Reading

Citations

Mar 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G RenaudR J Havel
Aug 1, 1991·The Journal of Investigative Dermatology·M F te PasM Ponec
Jun 1, 1992·The Journal of Investigative Dermatology·M PonecB J Vermeer
Mar 1, 1990·Journal of Dermatological Science·B J Vermeer, J A Leuven

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