Binding capacity of a barley beta-D-glucan to the beta-glucan recognition molecule dectin-1

Journal of Agricultural and Food Chemistry
Rui TadaNaohito Ohno

Abstract

To clarify whether barley beta-glucans exhibit their biological effects via binding to dectin-1, a pivotal receptor for beta-1,3-glucan, the structure of barley beta-glucan E70-S (BBG-70) was unambiguously investigated by NMR spectroscopy and studied for its binding capacity and specificity to dectin-1 by ELISA. NMR spectroscopy confirmed that BBG-70 contains two different linkage glucans, namely, alpha-glucan and beta-glucan, which are not covalently attached to one another. Beta-glucan within BBG-70 is a linear mixed-linkage beta-glucan composed of 1,3- and 1,4-beta-D-glucopyranose residues but does not contain the continuous 1,3-linkage. Competitive ELISA revealed that highly purified barley beta-glucan E70-S (pBBG-70) inhibits the binding of soluble dectin-1 to sonifilan (SPG), a beta-1,3-glucan, although at a concentration higher than that of SPG and laminarin. It was found that barley beta-glucan can be recognized by dectin-1, implying that barley beta-glucan might, at least in part, exhibit its biological effects via the recognition by dectin-1 of the ligand sugar structure, which may be formed by 1,3-beta- and 1,4-beta-glucosyl linkage.

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Citations

Dec 31, 2010·Applied Microbiology and Biotechnology·Solomon P Wasser
Aug 30, 2011·Glycobiology·Ryo KakutaniNaohito Ohno
Feb 23, 2011·International Journal of Molecular Sciences·Anne Berit SamuelsenSvein H Knutsen
Jan 9, 2009·Bioscience, Biotechnology, and Biochemistry·Minoru UjitaHiroki Okumura
May 30, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laurent LegentilVincent Ferrières
Feb 9, 2013·Bioscience, Biotechnology, and Biochemistry·Shingo HinoTatsuya Morita
Aug 11, 2012·International Immunopharmacology·Daisuke YamanakaNaohito Ohno
Dec 17, 2008·Annals of the New York Academy of Sciences·S Vicky Tsoni, Gordon D Brown
Apr 13, 2012·Molecular Nutrition & Food Research·Anne Rieder, Anne Berit Samuelsen
Sep 24, 2015·Molecular Nutrition & Food Research·Neha M SahasrabudhePaul de Vos
Mar 2, 2018·Journal of Nutritional Science and Vitaminology·Ayaka NakashimaKengo Suzuki
Apr 23, 2020·Animals : an Open Access Journal From MDPI·Vladimir Zmrhal, Petr Slama
Mar 7, 2021·International Journal of Molecular Sciences·Takashi KannoYoshiyuki Adachi
May 15, 2012·Bioorganic & Medicinal Chemistry·Hiroshi TanakaTakashi Takahashi
Sep 14, 2020·Allergology International : Official Journal of the Japanese Society of Allergology·Takashi KannoNaohito Ohno
Jun 13, 2017·International Journal of Biological Macromolecules·Yuina IshimotoNaohito Ohno
Oct 21, 2017·International Journal of Biological Macromolecules·Yuina IshimotoNaohito Ohno
Oct 9, 2019·Carbohydrate Polymers·Atsushi Miyagawa, Hatsuo Yamamura

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