Binding capacity of alpha-crystallin to bovine lens lipids

Experimental Eye Research
D Borchman, D Tang

Abstract

Three experiments were performed to determine the alpha-crystallin binding capacity of bovine lens lipid vesicles. In one experiment lipid was kept constant (2.5 mg ml-1) and the alpha-crystallin concentration was changed (0.5 to 3.0 mg ml-1). In another experiment, alpha-crystallin was kept constant (1 mg ml-1) and the concentration of lipid was varied (0.25 to 3 mg ml-1). We calculated the binding capacity of the lipid to be 0.33 +/- 0.05 (S.D.) mg alpha-crystallin (mg lens lipid)-1. This was confirmed by changes in the anisotropy and fluorescent intensity of a probe that partitions at the headgroup region of the lipid bilayer. Near 0.33 mg alpha-crystallin (mg lens lipid)-1 the fluorescence intensity and anisotropy of the probe increases and plateaus which indicates that concomitant with alpha-crystallin binding, water is excluded from the head group region of the bilayer and the headgroup region becomes less mobile. It is possible that alpha-crystallin binding could protect and stabilize the lipid bilayer and decrease membrane permeability.

Citations

Feb 29, 2000·The Journal of Biological Chemistry·B A Cobb, J M Petrash
Apr 22, 2010·Journal of Lipid Research·Douglas Borchman, Marta C Yappert
Apr 5, 2016·Experimental Eye Research·Stephen Barnes, Roy A Quinlan
Jan 20, 2005·Experimental Eye Research·P Farnsworth, Kamalendra Singh
Jan 7, 2009·Eye & Contact Lens·Douglas BorchmanJim Bell
Mar 13, 1999·Current Eye Research·Z ZhangC A Paterson
Jul 25, 2019·Cell Stress & Chaperones·Antonio De MaioWilbert Boelens
Jun 23, 2020·Current Eye Research·Laxman MainaliRaju Timsina
Nov 1, 2020·Experimental Eye Research·Raju TimsinaLaxman Mainali
Aug 14, 1998·Experimental Eye Research·D Tang, D Borchman
Jun 22, 2005·Experimental Eye Research·Vahid GramiDouglas Borchman
Jul 31, 2007·Biochimica Et Biophysica Acta·Justyna WidomskaWitold K Subczynski
Sep 4, 2021·Cell Stress & Chaperones·Antonio De Maio, Lawrence Hightower

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