PMID: 11343801May 10, 2001Paper

Binding features of chloroplast fructose-1,6-bisphosphatase-thioredoxin interaction

Biochimica Et Biophysica Acta
O S WangensteenJ López Gorgé

Abstract

It has been proposed that a hydrophobic groove surrounded by positively charged amino acids on thioredoxin (Trx) serves as the recognition and docking site for the interaction of Trx with target proteins. This model for Trx-protein interactions fits well with the Trx-mediated fructose-1,6-bisphosphatase (FBPase) activation, where a protruding negatively charged loop of FBPase would bind to this Trx groove, in a process involving both electrostatic and hydrophobic interactions. This model facilitates the prediction of Trx amino acid residues likely to be involved in enzyme binding. Site-directed mutagenesis of some of these amino acids, in conjunction with measurements of the FBPase activation capacity of the wild type and mutated Trxs, was used to check the model and provided evidence that lysine-70 and arginine-74 of pea Trx m play an essential role in FBPase binding. The binding parameters for the interaction between chloroplast FBPase and the wild type pea Trxs f and m, as well as mutated pea Trx m, determined by equilibrium dialysis in accordance with the Koshland-Nemethy-Filmer model of saturation kinetics, provided additional support for the role of these basic Trx residues in the interaction with FBPase. These data, in c...Continue Reading

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Citations

Apr 14, 2007·Current Genetics·Stéphane D LemaireEmmanuelle Issakidis-Bourguet
Sep 12, 2009·The Journal of Biological Chemistry·Jung-Sung ChungDavid B Knaff
Mar 11, 2003·Antioxidants & Redox Signaling·Peter Schürmann
Apr 2, 2008·Antioxidants & Redox Signaling·Peter Schürmann, Bob B Buchanan
Mar 28, 2009·Journal of Experimental Botany·Antonio Jesús SerratoMariam Sahrawy
Dec 18, 2002·Annals of the New York Academy of Sciences·Nicolas RouhierJean-Pierre Jacquot
Sep 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric GelhayeJean-Pierre Jacquot
Feb 18, 2009·Plant, Cell & Environment·Antonio Jesús SerratoMariam Sahrawy
Oct 11, 2019·The Journal of Biological Chemistry·Yuichi YokochiToru Hisabori
Jun 17, 2008·The Journal of Biological Chemistry·Cha San KohEric Gelhaye
Dec 18, 2002·Annals of the New York Academy of Sciences·Jean-Pierre JacquotEric Gelhaye

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