Binding-induced DNA walker for signal amplification in highly selective electrochemical detection of protein

Biosensors & Bioelectronics
Yuhang JiHuangxian Ju

Abstract

A binding-induced DNA walker-assisted signal amplification was developed for highly selective electrochemical detection of protein. Firstly, the track of DNA walker was constructed by self-assembly of the high density ferrocene (Fc)-labeled anchor DNA and aptamer 1 on the gold electrode surface. Sequentially, a long swing-arm chain containing aptamer 2 and walking strand DNA was introduced onto gold electrode through aptamers-target specific recognition, and thus initiated walker strand sequences to hybridize with anchor DNA. Then, the DNA walker was activated by the stepwise cleavage of the hybridized anchor DNA by nicking endonuclease to release multiple Fc molecules for signal amplification. Taking thrombin as the model target, the Fc-generated electrochemical signal decreased linearly with logarithm value of thrombin concentration ranging from 10pM to 100nM with a detection limit of 2.5pM under the optimal conditions. By integrating the specific recognition of aptamers to target with the enzymatic cleavage of nicking endonuclease, the aptasensor showed the high selectivity. The binding-induced DNA walker provides a promising strategy for signal amplification in electrochemical biosensor, and has the extensive applications i...Continue Reading

Citations

Feb 19, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rashaad A HusainZong-Hong Lin
Jul 22, 2020·Physical Review. E·David Arredondo, Darko Stefanovic
Dec 14, 2018·Chemical Communications : Chem Comm·Juan SongZhenyu Lin
Jul 31, 2020·Analytical Methods : Advancing Methods and Applications·Ying ChenXia Zuo
Jul 28, 2020·Advanced Science·Yaqin HuYanfei Liu
Nov 5, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Krishna ThapaRisheng Wang
Nov 9, 2021·Cancer Science·Tong MoXiaoyuan Chen

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