Binding Interactions of Keratin-Based Hair Fiber Extract to Gold, Keratin, and BMP-2

PloS One
Roche C de GuzmanMark E Van Dyke

Abstract

Hair-derived keratin biomaterials composed mostly of reduced keratin proteins (kerateines) have demonstrated their utility as carriers of biologics and drugs for tissue engineering. Electrostatic forces between negatively-charged keratins and biologic macromolecules allow for effective drug retention; attraction to positively-charged growth factors like bone morphogenetic protein 2 (BMP-2) has been used as a strategy for osteoinduction. In this study, the intermolecular surface and bulk interaction properties of kerateines were investigated. Thiol-rich kerateines were chemisorbed onto gold substrates to form an irreversible 2-nm rigid layer for surface plasmon resonance analysis. Kerateine-to-kerateine cohesion was observed in pH-neutral water with an equilibrium dissociation constant (KD) of 1.8 × 10(-4) M, indicating that non-coulombic attractive forces (i.e. hydrophobic and van der Waals) were at work. The association of BMP-2 to kerateine was found to be greater (KD = 1.1 × 10(-7) M), within the range of specific binding. Addition of salts (phosphate-buffered saline; PBS) shortened the Debye length or the electrostatic field influence which weakened the kerateine-BMP-2 binding (KD = 3.2 × 10(-5) M). BMP-2 in bulk kerateine ...Continue Reading

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Citations

Feb 24, 2016·Journal of Drug Delivery·Roche C de Guzman, Sina Y Rabbany
Feb 9, 2017·Tissue Engineering. Part a·J A PassipieriGeorge J Christ
May 13, 2017·Journal of Biomedical Materials Research. Part a·M N BiaoB C Yang
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Jul 22, 2019·Colloids and Surfaces. B, Biointerfaces·Alexis Trent, Mark E Van Dyke
Apr 9, 2018·ACS Biomaterials Science & Engineering·Nils A Potter, Mark Van Dyke

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Methods Mentioned

BETA
surface plasmon resonance
Protein Assay
gel filtration
electrophoresis
X-ray
chips
chip
Protein
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

ExPASy Proteomics server
PowerPoint
Fityk
Photoshop
Universal Protein Knowledgebase (
Multipak
Office Excel
Prism
GraphPad

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