PMID: 6984345Dec 30, 1982Paper

Binding, internalization and degradation of colony-stimulating factor by peritoneal exudate macrophages

Biochimica Et Biophysica Acta
B D ChenH S Lin

Abstract

Iodinated colony-stimulating factor produced by L-cells (125I-CSF-1) binds specifically to murine peritoneal exudate macrophages. At 37 degrees C, the cell-bound 125I-CSF-1 was internalized and degraded very rapidly, with the appearance of radioactive iodotyrosine in the medium. At 0 degree C, the cell-bound 125I-CSF-1 was not internalized and degraded, nor did it dissociate from the membrane. The internalization and degradation at 37 degrees C could be blocked or reduced by the presence of phenylglyoxal, methylamine and NH4Cl. The chemical nature of the CSF-1 binding site is polypeptide as judged by its sensitivity to trypsin treatment. After the binding and degradation of unlabeled CSF-1, the exudate cells were no longer able to rebind freshly added 125I-CSF-1, indicating the removal of CSF-1 binding site. The binding capacity of these cells, however, could be restored by prolonged incubation at 37 degrees C but not at 0 degrees C in culture medium containing fetal calf serum.

References

Aug 1, 1977·Journal of Cellular Physiology·T J MacVittie, K F McCarthy
Jun 1, 1979·Experimental Cell Research·D M ChenE R Stanley
Oct 1, 1975·The Journal of Experimental Medicine·H S LinT Kuo
Jun 1, 1974·Journal of Cellular Physiology·H S Lin, C C Stewart
May 5, 1981·Biochimica Et Biophysica Acta·Y YardenJ Schlessinger
Apr 1, 1980·The Journal of Cell Biology·L J Guilbert, E R Stanley

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Citations

Oct 15, 1987·International Journal of Cancer. Journal International Du Cancer·J Lotem, L Sachs
Jan 1, 1986·Journal of Cellular Biochemistry·L J GuilbertE R Stanley

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