PMID: 1319736Jul 1, 1992Paper

Binding of 111In-labeled HDL to platelets from normolipemic volunteers and patients with heterozygous familial hypercholesterolemia

Arteriosclerosis and Thrombosis : a Journal of Vascular Biology
I VirgoliniH Sinzinger

Abstract

High density lipoproteins (HDLs; d = 1.063 - 1.21 g/ml) were isolated by ultracentrifugation and radiolabeled with 111In. The in vitro binding onto platelets from healthy volunteers (n = 15) and patients (n = 36) with heterozygous familial hypercholesterolemia (FH) was investigated. Binding was saturable and indicated high-affinity binding sites, which bound 1,882 +/- 361 ng protein of 111In-HDL/10(9) platelets (dissociation constant [Kd] = 7 +/- 3 micrograms protein/ml) in healthy volunteers and significantly (p less than 0.01) lower amounts in the FH patients (1,012 +/- 439 ng protein of 111In-HDL/10(9) platelets [Kd = 12 +/- 4 micrograms protein/ml]; p less than 0.01). The capacity to displace one half of the bound ligand (IC50) amounted to 14 +/- 3 micrograms protein/ml in healthy volunteers and 22 +/- 9 micrograms protein/ml in FH patients (p less than 0.001). Treatment with lipid-lowering drugs (gemfibrozil, alone or in combination with cholestyramine) in 10 patients resulted in an increased HDL binding capacity: before treatment, 1,280 +/- 883; after 2 months of treatment, 2,052 +/- 873 (p less than 0.05); and after 6 months of treatment, 2,127 +/- 812 ng protein/10(9) platelets (p less than 0.01). There was a significan...Continue Reading

References

Jan 1, 1979·Lancet·E TremoliC Galli
Nov 30, 1978·The New England Journal of Medicine·A R Tall, D M Small
Nov 1, 1979·Atherosclerosis·K BergG Dahlen
Dec 15, 1977·The New England Journal of Medicine·J F Mustard, M A Packham
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·L H BlockM Regenass
Jul 1, 1988·Seminars in Thrombosis and Hemostasis·J G Brook, M Aviram
Dec 1, 1986·Journal of the American College of Cardiology·P L CannerW Friedewald
Feb 21, 1974·The New England Journal of Medicine·A C CarvalhoR S Lees
Apr 28, 1972·Nature·R I Hawkins
Jan 1, 1983·Acta Radiologica: Diagnosis·N HolstA Borgersen
Oct 1, 1981·European Journal of Biochemistry·J P TauberD Gospodarowicz
Apr 1, 1982·Hoppe-Seyler's Zeitschrift für physiologische Chemie·E KollerW Doleschel
Oct 15, 1983·The Biochemical Journal·D G HassallK R Bruckdorfer
Mar 1, 1983·Atherosclerosis·M Aviram, J G Brook
Jul 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·D G HassallB Lewis
Jan 1, 1980·Annual Review of Medicine·G J Miller
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON

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Citations

Mar 8, 2002·Atherosclerosis·Jerzy-Roch NoferArnold von Eckardstein
Sep 5, 2012·Thrombosis Journal·Helmut Sinzinger, Robert Berent
Jan 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·I VirgoliniP Valent
Apr 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·I VirgoliniH Sinzinger
Feb 24, 2007·Contrast Media & Molecular Imaging·Juan C FriasM Teresa Albelda

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