PMID: 6171531Nov 15, 1981Paper

Binding of 125I-labelled human alpha interferon to human lymphoid cells

International Journal of Cancer. Journal International Du Cancer
K E MogensenI Gressner

Abstract

To investigate the binding of interferon to human lymphoid cells, we purified human alpha interferon and radio-labelled it with iodine-125. Binding at 4 degrees C could be saturated and was inhibited by unlabelled interferon; it was specific for cells of human origin. Dissociation constants for the complex of interferon and receptor site were of the order 10(-9)-10(-11) M. All human cells tested showed such binding. Occupation of these high-affinity sites, at 37 degrees C, was compared with the inhibition of cellular growth due to interferon. The most sensitive cell line (Daudi) gave a complete biological response with only a fraction of its sites occupied. Evidence of two sites was found for a line (P3HRI) showing intermediate sensitivity. A relatively insensitive line (Raji) showed no response when all its high-affinity sites were occupied.

References

Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L D KohnG Lee
Jan 1, 1975·Journal of Clinical Microbiology·H StranderK Cantell
Aug 1, 1975·The Journal of General Virology·A AdamsK Cantell
Jan 1, 1976·Advances in Protein Chemistry·P Cuatrecasas, M D Hollenberg
Oct 27, 1978·Biochimica Et Biophysica Acta·I Gresser, M G Tovey
Oct 1, 1979·The Journal of General Virology·K E Mogensen, K Cantell
Nov 1, 1975·Blut·H U Schwenk, U Schneider
Jul 15, 1972·International Journal of Cancer. Journal International Du Cancer·G KleinB Gothoskar
Jan 1, 1967·Proceedings of the Society for Experimental Biology and Medicine·Y Hinuma, J T Grace
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·G KleinC Sundström
Dec 1, 1974·The Journal of General Virology·K CantellH Strander
Feb 1, 1974·Journal of the National Cancer Institute·I GresserD Brouty-Boyé
Feb 1, 1974·Virology·B Berman, J Vilcek
Jul 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·M G ToveyI Gresser
Feb 1, 1964·Lancet·J V PULVERTAFT

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Citations

Jan 1, 1987·Cancer Metastasis Reviews·M Shearer, J Taylor-Papadimitriou
Jan 1, 1984·Pharmacology & Therapeutics·K C Zoon, H Arnheiter
Jan 1, 1985·Pharmacology & Therapeutics·P B Fisher, S Grant
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P B FisherI B Weinstein
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C R FaltynekC Baglioni
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·F H Sarkar, S L Gupta
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A RaziuddinS L Gupta
Jan 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B L LiS Pestka
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O R ColamoniciR Nordan
Nov 5, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·K E MogensenG Uzé
Mar 25, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·P EidM G Tovey
Jan 1, 1984·Journal of Interferon Research·S L GuptaF H Sarkar
Jan 1, 1984·Journal of Interferon Research·N Morinaga, T Kuwata
Jan 1, 1984·Journal of Interferon Research·J Taylor-Papadimitriou, M Shearer
Jan 1, 1985·Journal of Interferon Research·B L MaxwellJ U Gutterman
Jan 1, 1985·Journal of Interferon Research·J A Langer, S Pestka
Jan 1, 1985·Journal of Interferon Research·S FeinsteinY Teitz
Apr 1, 1986·Journal of Interferon Research·J A LangerS Pestka
Jan 1, 1995·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·L E LingC D Benjamin
May 15, 1986·European Journal of Biochemistry·G E HanniganB R Williams
Feb 1, 1988·European Journal of Biochemistry·P EidK E Mogensen
May 30, 1983·FEBS Letters·P Eid, K E Mogensen
Jan 27, 1983·Biochemical and Biophysical Research Communications·G E HanniganB R Williams
Jun 15, 1984·Biochemical and Biophysical Research Communications·C Rochette-Egly, M G Tovey
Apr 1, 1985·European Journal of Cancer & Clinical Oncology·D Brouty-BoyéI Gresser
Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·N MorinagaT Kuwata
Jul 3, 2007·Cellular Signalling·Florence BaychelierPierre Eid

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