Binding of a pancreatic nuclear protein is correlated with amylase enhancer activity.

Nucleic Acids Research
G HowardM H Meisler

Abstract

The mouse amylase gene Amy-2.2 is expressed at high levels specifically in the acinar cells of the pancreas. The region between -172 and -110 of this gene includes sequence elements common to pancreas-specific genes. Nuclear proteins with specific affinity for this region were partially purified from rat pancreas. The consensus element of another pancreas-specific gene, elastase 1, competes for protein binding to the amylase sequences. Binding was localized by DNase I protection to the sequence -156 to -122. Site-directed mutagenesis of this sequence resulted in concomitant loss of protein binding and enhancer activity. Photo-affinity labelling of pancreatic nuclear extracts identified one predominant binding protein with a molecular weight of approximately 75 kDa. The data indicate that binding of this nuclear protein is essential for the enhancer activity of this pancreas-specific element.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·J G Sutcliffe
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A M BouletW J Rutter
Jun 5, 1987·Science·T ManiatisJ A Fischer
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T EvansG Felsenfeld
Jan 1, 1986·Annals of the New York Academy of Sciences·R J MacDonaldR L Brinster
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·U Siebenlist, W Gilbert

❮ Previous
Next ❯

Citations

May 1, 1994·Molecular and Cellular Endocrinology·P C Lee, X C Mao
Aug 1, 1993·The Proceedings of the Nutrition Society·I Le Huerou-LuronA Puigserver
Feb 17, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jun WangKen-ichi Yamamura
Sep 1, 1991·Molecular and Cellular Biology·K J WuG J Darlington
Dec 29, 1992·Biochimica Et Biophysica Acta·A CarsanaM Palmieri
Aug 1, 1995·Molecular and Cellular Biology·F KruseR J MacDonald

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.