PMID: 11607144Jan 1, 1991Paper

Binding of a single divalent cation directly correlates with the blue-to-purple transition in bacteriorhodopsin

Proceedings of the National Academy of Sciences of the United States of America
R Jonas, T G Ebrey

Abstract

We have characterized a unique divalent cation binding site on bacteriorhodopsin which controls the blue-to-purple transition in the purple membrane of Halobacterium halobium. To identify this site we first showed the correlation between the binding of one Ca2+ per bacteriorhodopsin and the amount of blue membrane converted to purple membrane. When the free Ca2+ was reduced below 1 microM, and the pH was set below 5.0 with 0.5 mM citrate, only binding to this high-affinity site was observed, and we could separate its effect from the effect of other divalent cations binding to the membrane under other conditions. Second, the titration of purple membrane showed that protons are taken up in two distinct steps, about 13 with a pKa of 4-5 and an additional 2 protons with a pKa of 2.75, in 5 mM MgSO4. The latter is identical to the pKa for the purple-to-blue transition in 5 mM MgSO4. Taken together, these observations strongly suggest a direct role for cations in the regulation of the bacteriorhodopsin color under normal conditions. We have also found that the intrinsic pKa for the purple-to-blue transition is about 2.05, suggesting this is the pKa of the group or groups that, when protonated, lead to the blue membrane. Previously pu...Continue Reading

References

Aug 1, 1979·Photochemistry and Photobiology·A Warshel, M Ottolenighi
Dec 15, 1979·Journal of Molecular Biology·D D Muccio, J Y Cassim
Nov 1, 1979·Biophysical Journal·U Fischer, D Oesterhelt
Dec 1, 1990·Photochemistry and Photobiology·R JonasT G Ebrey
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H OttoM P Heyn
Jun 20, 1990·Journal of Molecular Biology·R HendersonK H Downing
Feb 1, 1989·Trends in Biochemical Sciences·D Oesterhelt, J Tittor
Feb 1, 1986·Biophysical Journal·J L SpudichR A Bogomolni
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I Szundi, W Stoeckenius
Sep 29, 1971·Nature: New Biology·D Oesterhelt, W Stoeckenius
Nov 1, 1984·Photochemistry and Photobiology·Y KimuraW Stoeckenius
Oct 3, 1980·Biochimica Et Biophysica Acta·R Renthal, B Wallace
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C H ChangT Ebrey
Mar 1, 1986·Biophysical Journal·C H ChangT G Ebrey

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Citations

Jul 27, 2001·Journal of Photochemistry and Photobiology. B, Biology·G Wang, K S Hu
Dec 1, 1990·Photochemistry and Photobiology·R JonasT G Ebrey
Dec 8, 2009·Biochemistry. Biokhimii︠a︡·Yue ZhangKun-Sheng Hu
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·U HauptsD Oesterhelt
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·C RoselliM A el-Sayed
Aug 16, 2008·Proteins·Tsjerk A WassenaarAlan E Mark
Jul 19, 2005·Journal of Molecular Biology·Hideo OkumuraTsutomu Kouyama
Apr 25, 2015·Biochemistry·Sansa DuttaMordechai Sheves
Jul 9, 2004·Biophysical Journal·Francesc SepulcreEsteve Padrós
Jun 4, 1999·Biophysical Journal·G VáróJ K Lanyi
Jul 21, 2001·Biophysical Journal·T EliashM Sheves
Apr 6, 2017·The Journal of Physical Chemistry. B·Elena Smolensky KoganovMordechai Sheves
Feb 9, 1993·Biochemistry·G J TurnerR M Stroud
Dec 28, 1993·Biochemistry·N Y Zhang, M A el-Sayed
Oct 19, 2005·Chembiochem : a European Journal of Chemical Biology·Susana LópezEsteve Padrós
Nov 2, 2019·Nature Communications·Dmitry BratanovValentin Gordeliy
Jul 11, 2021·Journal of Computational Chemistry·Nicolas Frazee, Blake Mertz

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