Binding of an N-terminal rhodanese peptide to DnaJ and to ribosomes.

The Journal of Biological Chemistry
W KudlickiB Hardesty

Abstract

A peptide corresponding to the N-terminal 17 amino acids of bovine rhodanese was fluorescently labeled with a coumarin derivative at its primary amino group(s) and then purified by high performance liquid chromatography. This peptide interacted with the molecular chaperone DnaJ in the absence of other chaperones and ATP. In the presence of ATP, the molecular chaperone DnaK bound to the DnaJ-peptide complex, but not to the peptide alone. The chaperone GrpE appeared to cause the release of the peptide bound to the ternary complex in the presence of ATP but not in the presence of ADP. This nucleotide apparently stabilized the complex. The peptide also bound to salt-washed Escherichia coli 70 S ribosomes, specifically to 50 S ribosomal subunits, not to 30 S subunits. DnaJ plus DnaK interacted with the peptide on the ribosome. GrpE caused dissociation of the peptide from the ribosome; ATP was required for this reaction. It was inhibited by ADP. A comparable series of chaperone-mediated reactions is assumed to occur with the N-terminal segment of the nascent polypeptide to facilitate its folding on ribosomes.

References

May 11, 1978·Nature·J H PloegmanJ Russell
May 26, 1995·Journal of Molecular Biology·J S McCartyB Bukau
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·A N Fedorov, T O Baldwin
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·A SzaboF U Hartl
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J P HendrickM Wiedmann
Jan 21, 1994·Journal of Molecular Biology·A GragerovM E Gottesman

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Citations

May 30, 1998·Protein Science : a Publication of the Protein Society·D McCarthyB Hardesty
Aug 3, 2013·Current Microbiology·YingYing CaiXueMei Zhang
Jun 18, 2002·Bioorganic & Medicinal Chemistry Letters·Elisa PeroniAnna M Papini
Aug 28, 1999·Bioorganic & Medicinal Chemistry Letters·W Y LeungF Mao
Jul 29, 2010·The Journal of Biological Chemistry·Alexandre SmirnovIvan Tarassov
Aug 26, 1998·The EMBO Journal·W YanE A Craig
Apr 21, 1999·Protein Science : a Publication of the Protein Society·K HuangJ H Prestegard
Jan 31, 1998·The Journal of Biological Chemistry·A N Fedorov, T O Baldwin
Jun 20, 1998·The Journal of Biological Chemistry·B FeifelP Christen
Apr 18, 1998·The Journal of Biological Chemistry·E V PierpaoliP Christen
Mar 15, 2003·The Journal of Biological Chemistry·Pius BischofbergerPhilipp Christen
Apr 30, 1999·Physiological Reviews·A L Fink
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·P Fekkes, A J Driessen

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