Binding of apoA-IV-phospholipid complexes to plasma membranes of rat liver

Biochemical and Biophysical Research Communications
G GhiselliA M Gotto

Abstract

Rat apoA-IV complexes with dimyristoyl phosphatidylcholine (apoA-IV-DMPC) have been prepared and their ability to bind to purified rat liver plasma membranes investigated. Binding equilibrium at 37 degrees C was reached in 30 minutes. Saturation binding experiments and subsequent analysis of the results with Scatchard plots gave results consistent with the presence of a single saturable binding site. DMPC or POPC unilamellar vesicles could not compete with apoA-IV-DMPC for binding; apoA-I-DMPC competed only partially. ApoE-poor HDL effectively competed with apoA-IV-DMPC. The fact that binding could be greatly reduced (greater than 70%) by preincubating the membrane with pronase (18 micrograms/ml), supports the conclusion that a membrane protein is involved in binding. Based on these results, we speculate that the rapid catabolism of apoA-IV in plasma may be mediated by a specific uptake mechanism in the liver. The implications of these results support the hypothesis that apoA-IV is involved in reverse cholesterol transport.

References

Feb 1, 1978·The Journal of Clinical Investigation·P H GreenR M Glickman
Oct 26, 1978·The New England Journal of Medicine·E J SchaeferH B Brewer
Mar 28, 1986·Biochemical and Biophysical Research Communications·R B Weinberg, M S Spector
Sep 1, 1985·The Journal of Clinical Investigation·T OhtaP J Nestel
Apr 1, 1980·The Journal of Clinical Investigation·P H GreenE Quinet

❮ Previous
Next ❯

Citations

Nov 6, 1989·Biochimica Et Biophysica Acta·G GhiselliA M Gotto
May 22, 1990·Biochimica Et Biophysica Acta·R B Weinberg, C S Patton
Apr 24, 2008·The Journal of Biological Chemistry·Matthew R TubbW Sean Davidson
Sep 25, 1990·Nucleic Acids Research·P de TemmermanG Siest
Mar 31, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Go YoshimichiPatrick Tso
Nov 21, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Chun-Min LoPatrick Tso
Mar 1, 1988·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·N Savion, A Gamliel
Oct 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·A von EckardsteinG Assmann
Jul 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·A von EckardsteinG Assmann
Feb 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·A von EckardsteinG Assmann
Aug 22, 2000·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·M LefevreF L Greenway
Jul 20, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Chun Min LoPatrick Tso
Aug 28, 2013·Annals of Surgery·Marco RaffaelliGeltrude Mingrone
Sep 1, 1993·Nutrition Reviews·A H Merrill
Apr 3, 1989·Biochimica Et Biophysica Acta·C Y YangL Chan

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE Phenotypes

Apolipoprotein E (APOE) is a protein involved in fat metabolism and associated with the pathogenesis of Alzheimer's disease and cardiovascular disease. Here is the latest research on APOE phenotypes.

Related Papers

Biochimica Et Biophysica Acta
G GhiselliA M Gotto
Arteriosclerosis : an Official Journal of the American Heart Association, Inc
G M Dallinga-ThieA Van Tol
Proceedings of the National Academy of Sciences of the United States of America
Tobias SikosekErich Bornberg-Bauer
© 2021 Meta ULC. All rights reserved