Binding of avian IgY to type VII collagen does not activate complement and leucocytes and fails to induce subepidermal blistering in mice

The British Journal of Dermatology
Alina SesarmanCassian Sitaru

Abstract

Epidermolysis bullosa acquisita (EBA) is a severe autoimmune skin disease characterized by tissue-bound and circulating autoantibodies to type VII collagen, the major component of anchoring fibrils. When passively transferred into mice, rabbit IgG against type VII collagen induces Fc-dependent activation of complement, the recruitment of leucocytes into the skin, and subepidermal blistering. In addition to these inflammatory mechanisms, clinical and experimental evidence suggests that antibodies against type VII collagen might induce blisters by disrupting the ligand function of type VII collagen by an Fc-independent mechanism. To study noninflammatory mechanisms of blister formation in experimental EBA. We generated chicken IgY antibodies directed to recombinant type VII collagen and examined their pathogenic activity using ex vivo and animal models. Mice injected with these chicken IgY antibodies showed binding of the antibodies to the dermal-epidermal junction of skin sections. However, IgY antibodies did not fix complement C3 in enzyme-linked immunosorbent assay and immunofluorescence complement-binding assays. In addition, IgY antibodies did not induce dermal-epidermal separation ex vivo. Following their passive transfer i...Continue Reading

References

Nov 1, 1992·Journal of the American Academy of Dermatology·B R Smoller, D T Woodley
May 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M GassmannU Hübscher
Dec 1, 1985·The Journal of Investigative Dermatology·J R StanleyV Klaus-Kovtun
Dec 1, 1987·Veterinary Immunology and Immunopathology·H Ambrosius, D Hadge
Jan 1, 1971·Archives of Dermatology·H H RoenigkW F Bergfeld
Apr 19, 1984·The New England Journal of Medicine·D T WoodleyW R Gammon
Apr 1, 1981·The Journal of Investigative Dermatology·H YaoitaS I Katz
Nov 14, 2001·Clinics in Dermatology·D Hallel-HalevyD T Woodley
Mar 29, 2002·The Journal of Investigative Dermatology·Cassian SitaruDetlef Zillikens
Mar 31, 2004·Obstetrics and Gynecology·Cassian SitaruDetlef Zillikens
Jul 2, 2005·Journal of Dermatological Science·K Woźniak, C Kowalewski
Nov 9, 2005·Experimental Dermatology·Cassian Sitaru, Detlef Zillikens
Mar 18, 2006·The Journal of Investigative Dermatology·David T WoodleyMei Chen
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cassian SitaruDetlef Zillikens
Feb 6, 2007·Archives of Dermatological Research·Cassian SitaruDetlef Zillikens
Mar 24, 2007·The Journal of Pathology·M T ChiriacC Sitaru
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sidonia MihaiCassian Sitaru
May 24, 2007·Experimental Dermatology·Cassian Sitaru

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Citations

Jan 20, 2010·Cellular and Molecular Life Sciences : CMLS·Kinga CsorbaCassian Sitaru
Oct 17, 2015·Expert Review of Clinical Immunology·Ralf Ludwig
Dec 1, 2009·Dermatologic Clinics·Myung S Ko, M Peter Marinkovich
Nov 3, 2009·Journal of Cellular and Molecular Medicine·Gheorghe HundorfeanCassian Sitaru
Aug 6, 2014·Journal of Cellular and Molecular Medicine·Kinga CsorbaCassian Sitaru
Jun 2, 2012·Journal of Autoimmunity·Misa HiroseEnno Schmidt
Dec 17, 2014·The Journal of Experimental Medicine·Felix C WeberStefan F Martin
Jul 16, 2011·Molecular Immunology·Nina A DahaLeendert A Trouw
Jan 15, 2016·The Journal of Investigative Dermatology·Michael KasperkiewiczRalf J Ludwig
Apr 1, 2010·Drug Testing and Analysis·Elham A TahaShudong Wang
Jul 18, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lydia P van der SteenMarjolein van Egmond
Jul 16, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andreas ReckeRalf J Ludwig
Jan 29, 2019·Frontiers in Medicine·Hiroshi KogaKatja Bieber
Dec 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ana Gabriela SitaruCassian Sitaru
Sep 3, 2020·Vaccines·José M Pérez de la LastraAntonio Morales-delaNuez
May 1, 2021·Molecular Immunology·Carlos Leónidas LeivaPablo Chacana
Mar 28, 2019·Toxicon : Official Journal of the International Society on Toxinology·Carlos Leónidas LeivaPablo Chacana

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