Binding of bacterial peptidoglycan to CD14.

The Journal of Biological Chemistry
R DziarskiP S Tobias

Abstract

The hypothesis that soluble peptidoglycan (sPGN, a macrophage-activator from Gram-positive bacteria) binds to CD14 (a lipopolysaccharide (LPS) receptor) was tested. sPGN specifically bound to CD14 in the following three assays: binding of soluble 32P-CD14 (sCD14) to agarose-immobilized sPGN, enzyme-linked immunosorbent assay, and photoaffinity cross-linking. sCD14 also specifically bound to agarose-immobilized muramyl dipeptide or GlcNAc-muramyl dipeptide but not to PGN pentapeptide. Binding of sCD14 to both sPGN and ReLPS (where ReLPS is LPS from Salmonella minnesota Re 595) was competitively inhibited by unlabeled sCD14, 1-152 N-terminal fragment of sCD14, sPGN, smooth LPS, ReLPS, lipid A, and lipoteichoic acid but not by dextran, dextran sulfate, heparin, ribitol teichoic acid, or soluble low molecular weight PGN fragments. Binding of sCD14 to sPGN was slower than to ReLPS but of higher affinity (KD = 25 nM versus 41 nM). LPS-binding protein (LBP) increased the binding of sCD14 to sPGN by adding another lower affinity KD and another higher Bmax, but for ReLPS, LBP increased the affinity of binding by yielding two KD with significantly higher affinity (7.1 and 27 nM). LBP also enhanced inhibition of sCD14 binding by LPS, ReLP...Continue Reading

References

Jan 1, 1990·Annual Review of Biochemistry·C R Raetz
Dec 1, 1972·Bacteriological Reviews·K H Schleifer, O Kandler
Jul 1, 1974·Analytical Biochemistry·S C MarchP Cuatrecasas
Jul 1, 1983·European Journal of Biochemistry·W FischerR Haas
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·S J De KimpeJ R Vane
Dec 1, 1995·The Journal of Experimental Medicine·T KusunokiS D Wright
Jan 1, 1994·The Journal of Experimental Medicine·E HailmanS D Wright
Feb 1, 1994·Current Opinion in Immunology·R J Ulevitch, P S Tobias
Jan 6, 1995·The Journal of Biological Chemistry·S Viriyakosol, T N Kirkland
Jan 20, 1995·The Journal of Biological Chemistry·T S JuanH S Lichenstein
Mar 10, 1995·The Journal of Biological Chemistry·T S JuanH S Lichenstein
Sep 1, 1994·Immunity·J PuginR J Ulevitch
May 5, 1995·The Journal of Biological Chemistry·P S TobiasR J Ulevitch
Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·R L DeludeD T Golenbock
Jan 1, 1993·European Journal of Immunology·T EspevikA Sundan
Jan 1, 1994·Methods in Enzymology·R S Rosenthal, R Dziarski
Feb 1, 1996·Infection and Immunity·S Viriyakosol, T N Kirkland
Feb 23, 1996·The Journal of Biological Chemistry·B Yu, S D Wright
Sep 20, 1996·The Journal of Biological Chemistry·D GuptaR Dziarski
Jan 1, 1997·Infection and Immunity·R A ShapiroR P Darveau
Sep 12, 1997·The Journal of Biological Chemistry·R I Tapping, P S Tobias

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Citations

Mar 12, 2009·Molecules and Cells·Chunghyun LeeSang-Gi Paik
Jul 24, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Didier Heumann, Thierry Roger
Nov 18, 2000·Biochimica Et Biophysica Acta·R DziarskiD Gupta
May 26, 1999·Infectious Disease Clinics of North America·R R IngallsD Golenbock
Apr 18, 2003·Protein Expression and Purification·Shin-ichi NomuraKoichiro Takeshige
Dec 7, 2002·The Lancet Infectious Diseases·Uwe KoedelHans-Walter Pfister
Feb 4, 2012·Nature Reviews. Immunology·Clarissa C LeeHidde L Ploegh
Mar 17, 2007·Nature Reviews. Microbiology·Julien Royet, Roman Dziarski
Jan 29, 2000·European Journal of Clinical Investigation·P Antal-Szalmás
Sep 8, 2001·Mediators of Inflammation·D MuhvićD Rukavina
Oct 26, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D L HornJ B Zabriskie
Nov 26, 1999·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·A SediváH J Gabius
May 11, 2002·Critical Care Medicine·Philipp Henneke, Douglas T Golenbock
Feb 6, 2004·Critical Care Medicine·Mark T Lin, Timothy E Albertson
Jun 12, 2004·Critical Care Medicine·Sebastien Gibot
Mar 23, 2006·Shock·Anders E MyhreJacob E Wang
Aug 26, 2006·Science·Heather L CashLora V Hooper
Jul 16, 2005·Clinical Microbiology Reviews·Bénédicte Fournier, Dana J Philpott

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