Binding of bilirubin to mammalian erythrocytes

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
S Tayyab, M K Ali

Abstract

Binding of bilirubin to erythrocytes of various mammalian species in the presence of their respective plasma albumins was studied at pH 8.0, ionic strength 0.41 and at 37 degrees C. In human, buffalo, goat, and sheep, the amount of erythrocyte-bound bilirubin increased with the increase in both the bilirubin/albumin molar ratio (B/A) and the total bilirubin concentration. In all species, the binding patterns were qualitatively similar. However, at any given B/A, goat erythrocytes bound the highest amount of bilirubin, followed by buffalo and human erythrocytes; sheep erythrocytes bound the lowest amount of bilirubin. Increase in erythrocyte-bound bilirubin per unit increase in bilirubin concentration at a constant B/A as obtained from the values of the slope of the plot between erythrocyte-bound bilirubin and total bilirubin in the incubate, was found to be highest for goat erythrocytes, followed by buffalo, human, and sheep erythrocytes. At given bilirubin concentration, percentage fractional binding of bilirubin between any two B/As was found to be highest between 1.5 and 2.0, suggesting that a greater amount of bilirubin was transferred from plasma to cells between B/As 1.5 and 2.0. Percentage fractional binding of bilirubin...Continue Reading

References

Jan 31, 1990·Clinica Chimica Acta; International Journal of Clinical Chemistry·M HayerB M de Bornier
Feb 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation·D Bratlid
Sep 1, 1966·Transfusion·W H CheungH D Isenberg
Feb 15, 1983·The Biochemical Journal·H Sato, S Kashiwamata
Jan 1, 1980·CRC Critical Reviews in Clinical Laboratory Sciences·R Brodersen
Sep 30, 1980·Biochimica Et Biophysica Acta·Y Barenholz, T E Thompson
Dec 1, 1980·Pediatric Research·G JoriF F Rubaltelli
Jan 8, 1981·Clinica Chimica Acta; International Journal of Clinical Chemistry·A BouillerotF Sauger
Jul 1, 1995·Comparative Biochemistry and Physiology. Part A, Physiology·S Tayyab, M K Ali
Jan 1, 1958·Scandinavian Journal of Clinical and Laboratory Investigation·J FOG
Sep 1, 1962·Clinica Chimica Acta; International Journal of Clinical Chemistry·D WATSON
Nov 1, 1963·The Journal of Pediatrics·F A OSKI, J L NAIMAN
Jan 14, 2009·Pakistan Journal of Biological Sciences : PJBS·B Boon KimS Tayyab
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation·D Bratlid

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Citations

Jan 1, 2001·Indian Journal of Clinical Biochemistry : IJCB·M K AliS Tayyab
Jun 7, 2008·European Journal of Medicinal Chemistry·Athina GeronikakiGeorge Theophilidis
Oct 11, 2001·Journal of Perinatology : Official Journal of the California Perinatal Association·J M SteinkeA P Shepherd
Sep 1, 2005·Molecular and Cellular Biochemistry·Huma RashidSaad Tayyab
Feb 5, 2004·SAR and QSAR in Environmental Research·A GeronikakiJ C Dearden

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