PMID: 11909054Mar 23, 2002Paper

Binding of biological effectors on magnetic nanoparticles measured by a magnetically induced transient birefringence experiment

Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics
Claire WilhelmJ-C Bacri

Abstract

We have investigated the relaxation of the magnetically induced birefringence in a suspension of magnetic nanoparticles in order to detect the binding reaction of polyclonal antibodies on the particle surface. The birefringence relaxation is driven by the rotational diffusion of the complex formed by the magnetic nanoparticles bound to the antibody and thus is directly related to the hydrodynamic size of this complex. Birefringence relaxations are well described by stretched exponential laws revealing a polydisperse distribution of hydrodynamic diameters. Comparing the size distribution of samples with different initial ratios of immunoglobuline added per magnetic nanoparticles, we evidence the graft of an antibody on particle and eventually the onset of particles aggregation. Measurements on samples separated in size by gel filtration demonstrate the robustness of our experiment for the determination of size distribution and its modification due to the adsorption of a macromolecule. The immunoglobuline binding assay is performed comparatively for ionic magnetic nanoparticles with different coatings.

References

Dec 17, 1997·Journal of Colloid and Interface Science·N FauconnierA Bee
Feb 19, 1999·BioTechniques·A P KauschB D Bruce
Mar 4, 2000·Nature Medicine·R WeisslederJ P Basilion
Aug 5, 2000·Cytometry·K E McCloskeyM Zborowski
Nov 23, 2000·Bioconjugate Chemistry·D HögemannJ P Basilion

❮ Previous
Next ❯

Citations

Jun 8, 2010·Analytical and Bioanalytical Chemistry·Jason S Kim, Frances S Ligler
Oct 14, 2003·Journal of Colloid and Interface Science·Guillaume MériguetRégine Perzynski
Mar 15, 2005·Biomagnetic Research and Technology·Gilles K KouassiGregory McCarty
Jun 28, 2012·Nanomedicine·Bruno Teste, Stephanie Descroix
Mar 6, 2007·The European Physical Journal. E, Soft Matter·C RivièreR Perzynski
Apr 8, 2015·New Biotechnology·Donatien RamiandrisoaJean Baudry
Jun 9, 2016·Sensors·Stefan SchrittwieserJoerg Schotter
Jul 29, 2006·Lab on a Chip·Nicole Pamme, Claire Wilhelm
May 21, 2008·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Michael LévyFlorence Gazeau
Jan 16, 2007·Analytical Chemistry·Konstanze AurichWerner Weitschies

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.