PMID: 11934491Apr 6, 2002Paper

Binding of bovine lactoferrin to Streptococcus dysgalactiae subsp. dysgalactiae isolated from cows with mastitis

FEMS Microbiology Letters
Hee Myung ParkS P Oliver

Abstract

Three strains of Streptococcus dysgalactiae subsp. dysgalactiae (UT516, UT519, ATCC 27957) were used to determine if bovine lactoferrin (Lf) binds to bacterial cells by biotin avidin binding assay (BABA), enzyme-linked immunosorbent assay (ELISA), and binding inhibition assay. Binding assays revealed that all strains of S. dysgalactiae subsp. dysgalactiae (S. dysgalactiae) evaluated in this study bound to Lf. However, differences in Lf binding capability among strains and between methods used were detected. Binding of Lf was not inhibited by transferrin (Tf) and Lf moiety molecules (mannose, galactose, and lactose) but by Lf. This study demonstrates that S. dysgalactiae bound to bovine Lf in a specific manner.

References

Feb 1, 1976·Journal of Dairy Science·F K WeltyF L Schanbacher
Nov 1, 1992·FEMS Microbiology Letters·P Rainard
Apr 1, 1991·Journal of Dairy Science·A S NaiduJ L Watts
Sep 1, 1985·Journal of Dairy Science·D A TodhunterP S Schoenberger
Feb 1, 1994·Medical Hypotheses·M Rabinoff
Mar 14, 2000·American Journal of Veterinary Research·W FangS P Oliver

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Citations

Aug 8, 2021·Microorganisms·Tina KabelitzMarcus Fulde

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