Abstract
Metallothioneins (MTs) are a family of ubiquitous, low-molecular-mass, cysteine-rich proteins that play a significant role in maintaining intracellular metal homeostasis, eliminating metal toxification, and protecting cells against oxidative damages. Research activity on plant MTs, although known for 30 years, has only moderately increased in the past few years. In this study, a type 1 MT from maize (Zea mays) (ZmMT1) was successfully expressed in Escherichia coli strain BL21 (DE3). The UV absorption spectra recorded after the reconstitution of apo-ZmMT1 with different metals demonstrated that ZmMT1 can coordinate up to six Zn(II) ions, six Cd(II) ions, and even higher amounts of Pb(II). In addition, the general metal ion coordination abilities of ZmMT1 characterized by pH-dependent zinc-, lead- and cadmium-binding stability and by the competitive reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) were evaluated. Results showed that the affinity of metal ions for the recombinant form of ZmMT1 can be arranged as follows: Cd(II) > Pb(II) > Zn(II). The observation revealed that chelating agents, such as ethylene diamine tetraacetic acid (EDTA) and ATP, accelerate the oxidation of ZmMT1 in the following order: EDTA ≫ L-histi...Continue Reading
References
Mar 1, 1979·European Journal of Biochemistry·B E FischerH Sigel
Sep 23, 1991·FEBS Letters·A J de Framond
Nov 4, 1991·FEBS Letters·A M TommeyN J Robinson
Mar 12, 1990·FEBS Letters·I M EvansR R Croy
Feb 15, 1985·Analytical Biochemistry·S Damodaran
Apr 1, 1984·Analytical Biochemistry·T W ThannhauserH A Scheraga
May 12, 1981·Biochemistry·M VasákH A Hill
Jun 1, 1995·Plant Molecular Biology·C ChevalierP Raymond
Dec 1, 1993·Journal of Inorganic Biochemistry·M M SavasD H Petering
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·L J JiangB L Vallee
Oct 29, 1999·European Journal of Biochemistry·P K PanP C Huang
Mar 16, 2000·Proceedings of the National Academy of Sciences of the United States of America·L J JiangW Maret
Mar 15, 2001·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·B XingW Tang
Sep 12, 2002·Annual Review of Plant Biology·Christopher Cobbett, Peter Goldsbrough
Jun 29, 2005·Journal of Biochemical and Biophysical Methods·Gabriele MeloniMilan Vasák
Oct 22, 2005·Plant Molecular Biology·Anne Marie ZimeriRichard B Meagher
Oct 26, 2005·The Journal of Biological Chemistry·Wenke FengY James Kang
Nov 18, 2005·Aquatic Toxicology·J-C AmiardP S Rainbow
Jan 13, 2006·The Biochemical Journal·Fimmie ReineckeFrancois H Van der Westhuizen
Jan 11, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Estevão A Peroza, Eva Freisinger
Aug 19, 2007·Journal of the American Chemical Society·Artur Krezel, Wolfgang Maret
Jan 21, 2009·Dalton Transactions : an International Journal of Inorganic Chemistry·Eva Freisinger
Nov 1, 2009·Metallomics : Integrated Biometal Science·Xiaoqiong Wan, Eva Freisinger
Mar 26, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jens LoebusEva Freisinger
May 23, 2013·Metallomics : Integrated Biometal Science·Oksana I LeszczyszynClaudia A Blindauer
Nov 6, 2013·Molecular Biology Reports·Shalini MudalkarAttipalli Ramachandra Reddy
Dec 10, 2013·Metallomics : Integrated Biometal Science·Òscar PalaciosMercè Capdevila
Dec 3, 2014·International Journal of Biological Macromolecules·Azar ShahpiriMohammad Ali Asadollahi
Apr 11, 2015·Biochemical and Biophysical Research Communications·Yan LiWei Min Tian
Apr 16, 2016·International Journal of Biological Macromolecules·Soheil Pirzadeh, Azar Shahpiri