Binding of Cd(II), Pb(II), and Zn(II) to a type 1 metallothionein from maize (Zea mays)

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Lian DuanYue Sun

Abstract

Metallothioneins (MTs) are a family of ubiquitous, low-molecular-mass, cysteine-rich proteins that play a significant role in maintaining intracellular metal homeostasis, eliminating metal toxification, and protecting cells against oxidative damages. Research activity on plant MTs, although known for 30 years, has only moderately increased in the past few years. In this study, a type 1 MT from maize (Zea mays) (ZmMT1) was successfully expressed in Escherichia coli strain BL21 (DE3). The UV absorption spectra recorded after the reconstitution of apo-ZmMT1 with different metals demonstrated that ZmMT1 can coordinate up to six Zn(II) ions, six Cd(II) ions, and even higher amounts of Pb(II). In addition, the general metal ion coordination abilities of ZmMT1 characterized by pH-dependent zinc-, lead- and cadmium-binding stability and by the competitive reaction with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) were evaluated. Results showed that the affinity of metal ions for the recombinant form of ZmMT1 can be arranged as follows: Cd(II) > Pb(II) > Zn(II). The observation revealed that chelating agents, such as ethylene diamine tetraacetic acid (EDTA) and ATP, accelerate the oxidation of ZmMT1 in the following order: EDTA ≫ L-histi...Continue Reading

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Citations

Feb 3, 2021·Environmental Science and Pollution Research International·Anindita MitraDharmendra K Gupta
Apr 8, 2021·Plant Physiology and Biochemistry : PPB·James T Carrillo, Dulal Borthakur
Aug 28, 2021·Frontiers in Molecular Biosciences·Taylor R Cole, Tatyana I Igumenova

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