Abstract
CD44 is a major cell-surface receptor for hyaluronate (HA). By alternative RNA-splicing a large number of CD44 variants are generated. To explore the role of CD44 splicing in the regulation of cell binding to HA, three different isoforms of CD44 were transfected in the CD44 negative B-cell lymphoma line Namalwa and in the fibroblastoid cell line COS7. We observed that whereas the standard form of CD44 (CD44s) mediated adhesion of Namalwa to HA, Namalwa transfected with CD44v3-10 or CD44v8-10 was unable to bind to either immobilized or soluble HA. After stimulation of CD44 with an activating anti-CD44 mAb or with phorbol ester, the binding of CD44s to HA was 5- to 10-fold higher than that of the other two isoforms. By contrast, COS7 cells transfected with CD44s, CD44v8-v10, or CD44v3-v10 bound equally effectively to HA. Hence, in addition to alternative splicing, cell type determines CD44 binding to HA.
Citations
Mar 14, 2000·Cell Adhesion and Communication·M RochmanD Naor
Apr 12, 2003·Blood·Khalil A AzizMirko Zuzel
Jan 16, 2013·Oncogene·J ZeilstraS T Pals
Sep 13, 2013·American Journal of Physiology. Renal Physiology·Elena RampanelliSandrine Florquin
May 1, 1997·Journal of Cellular Physiology·N Iida, L Y Bourguignon
Feb 13, 2002·Leukemia·M Van DrielA C Bloem
Dec 10, 2002·Hybridoma and Hybridomics·S M DavernS J Kennel
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kasper M A RouschopSandrine Florquin
Jul 6, 2010·European Journal of Clinical Investigation·Pieter T BotImo E Hoefer
May 23, 2015·Frontiers in Immunology·Suniti MisraShibnath Ghatak
Jul 1, 1997·European Journal of Immunology·D R CroftI M Kramer
Oct 18, 2000·The Journal of Experimental Medicine·R van der VoortS T Pals
May 28, 2019·Frontiers in Immunology·Muhan LiuEva Turley
Mar 10, 2010·Arthritis and Rheumatism·José C CrispínKaren H Costenbader
Apr 29, 1998·Tissue Antigens·G KremmidiotisH Zola
Jun 30, 1998·European Journal of Immunology·R LiP Johnson
Feb 26, 1999·The Journal of Biological Chemistry·R van der VoortS T Pals
Nov 6, 2020·Pharmaceutical Research·Wangxi HaiYuhong Xu
Oct 23, 1997·Current Opinion in Cell Biology·P W KincadeL Hanson
Apr 6, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hyukjin LeeTae Gwan Park
Sep 4, 2021·The FEBS Journal·Qian GuoFeng Gao
Feb 10, 2009·Fertility and Sterility·Jason S GriffithRobert S Schenken
Dec 5, 2002·Genes & Development·Véronique Orian-RousseauHelmut Ponta