Binding of cholesterol by Neisseria gonorrhoeae.

Infection and Immunity
R D MillerS A Morse

Abstract

The binding of [1,2-3H]cholesterol to Neisseria gonorrhoeae CS-7, Pseudomonas aeruginosa, and Salmonella typhimurium (smooth and rough strains) was investigated. The kinetics of cholesterol binding to N. gonorrhoeae CS-7 demonstrated that binding occurred slowly with maximum binding by 10 h. Under optimum conditions, a large percentage (65%) of the added cholesterol was associated with the cells. Chemical fractionation revealed that ca. 98% of the labeled cholesterol was associated with the cell membrane(s). The bound cholesterol was not esterified and was associated primarily with the cytoplasmic membrane. Intact gonococci bound 4 to 30 times more cholesterol than the deep rough mutant S. typhimurium TA1535, the wild-type S. typhimurium DB-21, and P. aeruginosa. In contrast, isolated cell membranes from all organisms rapidly bound cholesterol to the same extent. Therefore, the outer membrane can function as a permeability barrier to cholesterol. Cholesterol binding to both whole cells and isolated cell membranes was influenced by the incubation temperature. The rate of cholesterol binding by whole cells of N. gonorrhoeae decreased markedly at lower temperatures, with almost complete cessation of binding at 0 degrees C. A simil...Continue Reading

References

Aug 1, 1977·Infection and Immunity·R D MillerS A Morse
Apr 1, 1977·Infection and Immunity·R D Miller, S A Morse
Oct 1, 1975·Journal of Bacteriology·S Razin
Feb 1, 1975·Analytical Biochemistry·U Fricke
Dec 1, 1974·Infection and Immunity·S A Morse, T J Fitzgerald
Nov 1, 1974·Journal of Bacteriology·S A MorseJ Hines
Apr 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·S A Morse, L Bartenstein
Sep 20, 1968·Archives of Biochemistry and Biophysics·A O StoppaniJ A Brignone
Feb 1, 1967·Journal of Bacteriology·F VarricchioA Stevens

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Citations

Aug 1, 1980·Antimicrobial Agents and Chemotherapy·P G Lysko, S A Morse

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