PMID: 11934566Apr 6, 2002Paper

Binding of Clostridium difficile to Caco-2 epithelial cell line and to extracellular matrix proteins

FEMS Immunology and Medical Microbiology
Marina CerquettiPaola Mastrantonio

Abstract

Adhesion of Clostridium difficile to Caco-2 was examined as a function of monolayers polarization and differentiation. The number of adherent C. difficile C253 bacteria per cell strongly decreased when postconfluent 15-day-old monolayers were used (1.7 bacteria per cell versus 17.3 with 3-day-old monolayers). Following disruption of intercellular junctions by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N',-tetraacetic acid, a significant rise in the level of bacterial adhesion was observed, above all in postconfluent monolayers. Immunofluorescence studies of bacteria and transferrin receptor, a marker of basolateral pole of polarized monolayers, showed that C. difficile C253 adheres mainly to the basolateral surface of differentiated and undifferentiated polarized Caco-2 cells. Furthermore, binding of C. difficile C253 to several extracellular matrix proteins in vitro was demonstrated by an ELISA-based assay.

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Citations

Dec 5, 2013·PloS One·Séverine PéchinéAnne Collignon
Sep 14, 2002·Infection and Immunity·Emanuela CalabiNeil F Fairweather
Sep 3, 2010·Molecular Microbiology·Birendra SinghKristian Riesbeck
May 16, 2008·Journal of Medical Microbiology·Paola BrunIgnazio Castagliuolo
May 19, 2012·Journal of Medical Microbiology·Daniel Paredes-Sabja, Mahfuzur R Sarker
Jul 23, 2014·Infection and Immunity·Dennis BakkerJeroen Corver
Jun 29, 2011·FEMS Immunology and Medical Microbiology·Séverine PéchinéAnne Collignon
Feb 4, 2014·FEMS Microbiology Reviews·Uwe B SleytrDietmar Pum
Jun 30, 2016·FEMS Microbiology Reviews·Erin B Purcell, Rita Tamayo
May 23, 2019·Frontiers in Microbiology·Blessing O AnonyeMeera Unnikrishnan
May 16, 2008·Journal of Medical Microbiology·Cécile DenèveClaire Janoir
Aug 24, 2012·Future Microbiology·Mahfuzur R Sarker, Daniel Paredes-Sabja
Jan 22, 2014·Journal of Medical Microbiology·C H ChiltonH N Shah
Jun 16, 2021·PloS One·Giuseppe Valerio De GaetanoConcetta Beninati
Oct 7, 2004·Pathologie-biologie·C JanoirA Collignon

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