Binding of corticotropin-releasing hormone (CRH) in maternal and fetal plasma and in amniotic fluid

Clinica Chimica Acta; International Journal of Clinical Chemistry
K Salminen-Lappalainen, T Laatikainen

Abstract

Placenta secretes corticotropin-releasing hormone (CRH) into the maternal and fetal circulation, but a CRH binding protein in plasma may decrease its biological activity. Using a charcoal adsorption method we found that 92% of added 125I-Tyr-CRH was bound to a binding protein in the nonpregnant plasma, 72% in the plasma at term pregnancy, 90% in umbilical cord plasma, 82% in the amniotic fluid in the second and 25% in the third trimester. CRH added to plasma inhibited the binding of 125I-Tyr-CRH over the concentration range of 0.1-8.8 nmol/l in plasma and of 0.1-2.2 nmol/l in amniotic fluid. There was a significant negative correlation (R = -0.80) between the binding capacity of the CRH-binding protein and CRH concentration in maternal plasma. Plasma or amniotic fluid was incubated with 125I-Tyr-CRH and subjected to gel filtration on Sephadex G-50. The bound radioactivity was eluted at the region of Mr 25-40 kDa and the unbound radioactivity at the location of synthetic CRH. Bound and unbound CRH concentrations were determined using charcoal adsorption method and gel filtration on Sephadex G-50 in ten maternal plasma samples at the third trimester of pregnancy. Following mean percentages were found to be bound: charcoal method ...Continue Reading

References

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Citations

Jul 4, 2013·Clinical Obstetrics and Gynecology·Charles E Wood
Jun 1, 1997·Endocrine Reviews·S Mesiano, R B Jaffe
Jan 9, 2007·BJOG : an International Journal of Obstetrics and Gynaecology·R RomeroM Mazor
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Dec 16, 1998·The Journal of Clinical Endocrinology and Metabolism·I M LeitchR Smith
Feb 13, 2001·European Journal of Clinical Investigation·B SaeedC Self

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