Binding of CTP:phosphocholine cytidylyltransferase to lipid vesicles: diacylglycerol and enzyme dephosphorylation increase the affinity for negatively charged membranes

Biochemistry
R S ArnoldR Cornell

Abstract

The regulation of membrane binding and activity of purified CDP:phosphocholine cytidylyl-transferase (CT) by lipid activators and enzyme dephosphorylation was examined. The binding of CT to membranes was analyzed using sucrose-loaded vesicles (SLVs). Binding to phosphatidylcholine vesicles was not detected even at a lipid:protein ratio of approximately 2000 (1 mM PC). CT bound to vesicles containing anionic lipids with apparent molar partition coefficients between 2 x 10(5) and 2 x 10(6), depending on the vesicle charge. The vesicle binding and activation of CT showed very similar sigmoidal dependencies on the lipid negative charge. In addition, diacylglycerol interacted synergistically with anionic phospholipids to stimulate both binding and activation at lower mole percent anionic lipid. These results demonstrate parallel requirements for binding and activity. Dephosphorylation of CT without destabilization was accomplished using the catalytic subunit of protein phosphatase 1. Dephosphorylated CT required a lower mole percent anionic phospholipid than phosphorylated CT for binding to and activation by SLVs. The combination of 10 mol % diacylglycerol and enzyme dephosphorylation shifted the mole percent phosphatidic acid requi...Continue Reading

Citations

Jul 9, 1999·Progress in Lipid Research·F M Goñi, A Alonso
Sep 6, 2000·Trends in Biochemical Sciences·R B Cornell, I C Northwood
Apr 2, 1999·Biochimica Et Biophysica Acta·W TangI Tabas
Sep 9, 1999·Biochimica Et Biophysica Acta·P SomerharjuK H Cheng
Apr 16, 2003·European Journal of Biochemistry·Patrik StormAke Wieslander
Dec 21, 2012·Journal of the Royal Society, Interface·Marcus K DymondGeorge S Attard
May 15, 2004·Molecular and Cellular Biology·Suzanne JackowskiMohammad A Karim
Mar 13, 2014·Annual Review of Biochemistry·Guillaume Drin
Aug 17, 1999·The Journal of Peptide Research : Official Journal of the American Peptide Society·K F LauP C Shaw
Jan 10, 2016·Biochimica Et Biophysica Acta·Rosemary B Cornell
Feb 13, 2008·Journal of Cellular and Molecular Medicine·Pablo V EscribáGwendolyn Barceló-Coblijn
Mar 21, 2016·Trends in Cell Biology·Nora KoryTobias C Walther
Mar 8, 2005·Biochimica Et Biophysica Acta·Claudia Kent
Feb 10, 2006·Archives of Biochemistry and Biophysics·Alan J RyanRama K Mallampalli
Jul 28, 2015·Developmental Cell·Nora KoryTobias C Walther
Nov 16, 2013·Zygote : the Biology of Gametes and Early Embryos·Rodrigo Fernández-BussyGabriela Coux
Dec 6, 2003·Journal of Lipid Research·Charles E ChalfantYusuf A Hannun
Mar 7, 2006·The Journal of Biological Chemistry·Alexander AranovichAbraham H Parola
May 29, 2020·Life Science Alliance·Jonghwa LeeNeale D Ridgway
Feb 9, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·J VivekanandaR J King
Jun 19, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·S AwasthiR J King
Nov 13, 2004·The Journal of Biological Chemistry·Suzanne Jackowski, Paolo Fagone
Jun 5, 1998·The Journal of Biological Chemistry·A LykidisS Jackowski
Sep 7, 2019·The Journal of Biological Chemistry·Svetla G TanevaRosemary B Cornell
Feb 18, 2021·Biology Open·Santosh KumarChristopher G Burd
Apr 3, 2020·Journal of Molecular Biology·Rosemary B Cornell
Apr 30, 2020·Progress in Lipid Research·Peter K LundquistRoberto Espinoza-Corral

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