PMID: 6164391Mar 31, 1981Paper

Binding of divalent cations of dipalmitoylphosphatidylcholine bilayers and its effect on bilayer interaction

Biochemistry
L J LisR P Rand

Abstract

We have confirmed that CaCl2 swells the multilayer lattice formed by dipalmitolyphosphatidylcholine (DPPC) in an aqueous solution. Specifically, at room temperature 1 mM CaCl2 causes these lipid bilayers to increase their separation, dw, from 19 A in pure water to greater than 90 A. CaCl2 concentrations greater than 4 mM cause less swelling. We have measured the net repulsive force between the bilayers in 30 mM CaCl2 at T = 25 degrees C (below the acyl chain freezing temperature). For interbilayer separations between 30 and 90 A, the dominant repulsion between bilayers is probably electrostatic; Ca2+ binds to DPPc lecithin bilayers, imparting a charge to them. The addition of NaCl to CaCl2 solutions decreases this repulsion. For dw less than 20 A, the bilayer repulsion appears to be dominated by the "hydration forces" observed previously between both neutral and charged phospholipids. From the electrostatic repulsive force, we estimate the extent of Ca2+ binding to the bilayer surface. The desorption and bound Ca2+, apparent when bilayers are pushed together, is more rapid than one would expect if an association constant governed Ca2+ binding. The association affinity does not appear to be a fixed quantity but rather a sensitiv...Continue Reading

References

Mar 17, 1977·Biochimica Et Biophysica Acta·D PapahadjopoulosR Lazo
Jan 1, 1979·Membrane Biochemistry·S J MorrisD H Haynes
Dec 1, 1979·Biophysical Journal·J StamatoffP Eisenberger
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·V A ParsegianR P Rand
Jun 13, 1979·Biochemical and Biophysical Research Communications·W F GraddickN Spielberg
Jul 25, 1978·Biochemistry·A C CowleyV A Parsegian
Nov 16, 1978·Biochimica Et Biophysica Acta·A McLaughlinS McLaughlin
May 1, 1977·Biophysical Journal·D M LeNeveu, R P Rand
Aug 1, 1977·Biochimica Et Biophysica Acta·H HauserR J Williams
Jul 3, 1975·Biochimica Et Biophysica Acta·J Lansman, D H Haynes
Nov 17, 1975·Biochimica Et Biophysica Acta·Y InokoT Mitsui
Feb 19, 1976·Nature·D M LeNeveuV A Parsegian
Dec 17, 1969·Biochimica Et Biophysica Acta·E Cubero Robles, D van den Berg

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Citations

Aug 5, 1991·Biochimica Et Biophysica Acta·P W SandersonW P Williams
Jun 18, 1991·Biochimica Et Biophysica Acta·L S Bakás, E A Disalvo
Mar 27, 1984·Biochimica Et Biophysica Acta·B J BensonT Sargeant
Jan 1, 1986·Chemistry and Physics of Lipids·W Tamura-LisL J Lis
Jan 1, 1986·Chemistry and Physics of Lipids·B A CunninghamL J Lis
Mar 1, 1988·Chemistry and Physics of Lipids·B A CunninghamL J Lis
Feb 1, 1988·Chemistry and Physics of Lipids·G PifatJ N Herak
Mar 1, 1991·Chemistry and Physics of Lipids·G Cevc
Oct 8, 1990·Biochimica Et Biophysica Acta·G Cevc
Jan 1, 1987·Magnetic Resonance Imaging·G D FullertonI L Cameron
Apr 7, 1998·Biochimica Et Biophysica Acta·S Tristram-NagleJ F Nagle
Nov 1, 1985·Quarterly Reviews of Biophysics·K D Collins, M W Washabaugh
May 24, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Or SzekelyUri Raviv
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D C RauV A Parsegian
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E A Evans, V A Parsegian
Jul 1, 1984·The Journal of Cell Biology·V A Parsegian, D C Rau
May 1, 1990·The Journal of Pharmacy and Pharmacology·C A BrearleyC J Olliff
Sep 17, 2009·The European Physical Journal. E, Soft Matter·R Gerami, R F Bruinsma
Feb 19, 2010·The European Physical Journal. E, Soft Matter·Y S JhoP A Pincus
May 6, 2011·Colloids and Surfaces. B, Biointerfaces·Adrián LengyelPavol Balgavý
Sep 28, 2014·Biochimica Et Biophysica Acta·Ziad KhattariTim Salditt
Nov 13, 2010·Colloids and Surfaces. B, Biointerfaces·M J Santander-OrtegaJ L Ortega-Vinuesa
Sep 28, 2010·Journal of Colloid and Interface Science·Timothy A OlesonJoel A Pedersen
Feb 23, 2012·Scientific Reports·Maik HadornPeter Eggenberger Hotz
Mar 19, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Anna Jakubowska
May 9, 1990·Biochimica Et Biophysica Acta·G L JendrasiakR S Porter
Jun 26, 2007·Biophysical Journal·Georg PabstPeter Laggner
Jan 1, 1983·Annals of the New York Academy of Sciences·V A Parsegian, R P Rand
Nov 18, 1991·Biochimica Et Biophysica Acta·S A TatulianA G Syrykh
Dec 21, 1983·Biochimica Et Biophysica Acta·J F HahnL J Lis
Jun 19, 2013·Biochimica Et Biophysica Acta·Guido AngeliniCarla Gasbarri
Mar 12, 2015·Physical Chemistry Chemical Physics : PCCP·Jinsuk SongSongi Han
Jan 17, 2012·Journal of Colloid and Interface Science·Timothy A OlesonAnthony J Giuffre

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