Binding of dynein intermediate chain 2 to paxillin controls focal adhesion dynamics and migration.

Journal of Cell Science
Carine RossePeter J Parker

Abstract

In migrating NRK cells, aPKCs control the dynamics of turnover of paxillin-containing focal adhesions (FA) determining migration rate. Using a proteomic approach (two-dimensional fluorescence difference gel electrophoresis), dynein intermediate chain 2 (dynein IC2) was identified as a protein that is phosphorylated inducibly during cell migration in a PKC-regulated manner. By gene silencing and co-immunoprecipitation studies, we show that dynein IC2 regulates the speed of cell migration through its interaction with paxillin. This interaction is controlled by serine 84 phosphorylation, which lies on the aPKC pathway. The evidence presented thus links aPKC control of migration to the dynein control of FA turnover through paxillin.

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Citations

Jul 19, 2017·ELife·William B RedwineSamara L Reck-Peterson
Apr 25, 2018·Biology Open·Claudia ConteEric R Griffis
Dec 21, 2019·Biology of the Cell·Marta FructuosoDenis Dujardin
May 8, 2018·Scientific Reports·Rosa FontanaMaria Vivo
Feb 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·Tomas RojasChenglai Fu
Apr 18, 2018·Nature Reviews. Molecular Cell Biology·Samara L Reck-PetersonAndrew P Carter
Jul 13, 2014·Journal of Cell Science·Luis Calderilla-BarbosaMichael C Wooten

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