Binding of enterotoxigenic Escherichia coli to isolated enterocytes and intestinal mucus

Microbial Pathogenesis
A HelanderA M Svennerholm

Abstract

Binding of human enterotoxigenic Escherichia coli (ETEC) to the small intestine is a prerequisite for colonization and is mediated by colonization factor (CF) antigens. Coli surface antigen 6 (CS6) is considered a CF but binding to isolated enterocytes has not been established. In this study bacteria expressing CS6 were analysed for binding to enterocytes from human and rabbit small intestine, isolated using either an EDTA-containing buffer or a buffer devoid of EDTA. We found that the bacteria bound to enterocytes from rabbit ileum and human duodenum, but only when the cells had been isolated in the absence of EDTA. Pretreatment of rabbit enterocytes with meta-periodate resulted in a decreased proportion of cells with bound bacteria. Purified CS6, and for comparison other ETEC CFs, were also tested for binding to different human and rabbit mucus fractions. These analyses showed that purified CS6 bound to mucus from rabbit duodenum and ileum as well as from human duodenum, jejunum and ileum and that this binding was abolished by pretreatment of the mucus material with meta-periodate or Proteinase K. CFA/I, CS1 to CS5, CS7, CS17, putative CF (PCF) O159 (CS12), PCFO166 (CS14), and CFA/III (CS8) also bound to the rabbit mucus mate...Continue Reading

References

Sep 1, 1991·Journal of Clinical Microbiology·N BinszteinA M Svennerholm
Aug 31, 1990·Biochemical and Biophysical Research Communications·J R GumY S Kim
Jun 1, 1985·Infection and Immunity·S KnuttonA S McNeish
Dec 1, 1985·The Journal of Infectious Diseases·L GotheforsA M Svennerholm
Jan 1, 1982·Advances in Experimental Medicine and Biology·I CarlstedtL A Fransson
Mar 1, 1995·European Journal of Biochemistry·J MauryS Maroux
Jan 14, 1994·Biochemical and Biophysical Research Communications·G C HanssonK Klinga-Levan
Nov 1, 1996·Trends in Microbiology·W Gaastra, A M Svennerholm

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Citations

Nov 20, 2002·Infection and Immunity·Fatema NomanbhoyPaul L Fidel
Mar 16, 2002·Infection and Immunity·Fernando Güereña-BurgueñoGregory M Glenn
Jun 29, 2007·Journal of Clinical Microbiology·Abhisek GhosalNabendu Sekhar Chatterjee
Dec 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Sean-Paul Nuccio, Andreas J Bäumler
Jun 28, 2005·The International Journal of Biochemistry & Cell Biology·Jayasree BasivireddyKunissery A Balasubramanian
Dec 28, 2010·European Journal of Pharmacology·Nageswaran SivalingamMolly Jacob

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