Binding of Escherichia coli heat-stable enterotoxin to rat intestinal cells and brush border membranes.

Infection and Immunity
J C FrantzD C Robertson

Abstract

The association of heat-stable enterotoxin (STa) produced by enterotoxigenic Escherichia coli 431 with isolated rat intestinal epithelial cells and brush border membranes was characterized. Specific binding of strain 431 125I-STa to a single class of specific high-affinity receptors was saturable and temperature dependent and reached a maximum between 5 and 10 min. A 1,000-fold excess of unlabeled 431 STa competitively displaced 90 to 95% of radiolabeled enterotoxin bound to brush border membranes. In contrast, specific binding of 431 125I-STa to intestinal cells ranged from 40 to 65%. The number of STa-specific receptors on rat intestinal cells determined by Scatchard analysis was 47,520 +/- 14,352 (mean +/- standard error of the mean) per cell, with affinity constants (KaS) of 2.55 X 10(11)and 4.32 x 10(11) liters/mol determined for intestinal cells and brush border membranes, respectively. Villus intestinal cells appeared to possess about twice as many STa receptors as did crypt cells. Dissociation of specifically bound 431 125I-STa from intestinal cells and brush border membranes was minimal (2 to 5%). In addition, neither the rate nor the extent of dissociation was increased by a 1,000-fold excess of unlabeled homologous 4...Continue Reading

References

Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M FieldP L Smith
Jan 1, 1979·Annual Review of Biochemistry·J Moss, M Vaughan
Sep 1, 1980·Infection and Immunity·R N GreenbergR L Guerrant
Mar 1, 1983·The Journal of Infectious Diseases·D D Thomas, F C Knoop
Dec 15, 1982·European Journal of Biochemistry·S AimotoT Miwatani
Dec 1, 1982·Analytical Biochemistry·R LallierS A St-Pierre
Nov 18, 1980·Biochimica Et Biophysica Acta·H HauserD E Bowyer
Sep 1, 1981·Infection and Immunity·D M GillR A Finkelstein

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Citations

Sep 28, 1989·The New England Journal of Medicine·M FieldE B Chang
May 20, 1998·Journal of Pediatric Gastroenterology and Nutrition·A Fasano
Mar 1, 1992·Pharmacology & Toxicology·A A LimaM C Fonteles
Oct 1, 1990·Pflügers Archiv : European journal of physiology·G T McEwanE Skadhauge
Apr 13, 1999·The American Journal of Physiology·A Fasano
Nov 1, 1993·Infection and Immunity·B W Carpick, J Gariépy
Sep 1, 1991·Journal of Clinical Microbiology·R G UrbanL A Dreyfus
May 1, 1984·Infection and Immunity·L A DreyfusD C Robertson
Feb 28, 1989·Biochemical and Biophysical Research Communications·A A WhiteL R Forte

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