PMID: 8939794Jan 1, 1997Paper

Binding of extracellular matrix proteins by enterococci

Current Microbiology
T W ZarebaT Wadström

Abstract

Forty-four enterococcal strains isolated from human clinical specimens were investigated for binding of 125I-labeled fibronectin, vitronectin, thrombospondin, lactoferrin, and collagen type I and IV, and for cell surface hydrophobicity. Most strains expressed low binding of iodine-labeled human fibronectin, collagen I and IV, and higher binding of human vitronectin, human lactoferrin, and human thrombospondin. Bacteria grown in Todd-Hewitt broth exhibited increased binding to vitronectin and thrombospondin. In particle agglutination assays (PAA), Enterococcus faecalis strains reacted strongly with coated latex beads in contrast to E. faecium strains, which generally did not react. The ability of enterococci to bind ECM proteins was affected by heating and proteolytic digestion, suggesting that some protein-binding components become surface exposed after treatment with proteases. The binding of 125I-labeled proteins to E. faecalis strain E70 was inhibited when cells were preincubated with unlabeled proteins. Preincubating cells with sulfated polymers such as dextran sulfate (Mr 5000 and 8000), pentosan sulfate and heparin decreased binding of vitronectin, lactoferrin, and thrombospondin. The binding of lactoferrin and thrombospo...Continue Reading

Citations

Jun 5, 1999·International Journal of Food Microbiology·C M FranzM E Stiles
Mar 20, 2002·Research in Microbiology·Christine ArchimbaudBernard Joly
Nov 3, 2001·International Journal of Antimicrobial Agents·E C Leitch, M D Willcox
Feb 23, 2002·Trends in Cell Biology·Tzvi Tzfira, Vitaly Citovsky
Sep 11, 2013·Bioscience, Biotechnology, and Biochemistry·Shengbin RaoFumiki Morimatsu
Sep 3, 2010·Molecular Microbiology·Birendra SinghKristian Riesbeck
Aug 11, 2010·FEMS Microbiology Reviews·Brian HendersonMark A Williams
Dec 28, 2002·Microbiological Research·Igor StyriakAsa Ljungh
Jan 10, 2018·Polish Journal of Microbiology·Paweł Lisiecki
Jul 17, 2004·Microbiology·Jouko SillanpääMagnus Höök
Sep 10, 1999·The Journal of Biological Chemistry·R L RichM Höök
Sep 30, 1998·FEMS Immunology and Medical Microbiology·J XiaoB E Murray

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