Binding of fibrinogen to human monocytes

The Journal of Clinical Investigation
D C AltieriA M Capitanio

Abstract

The interaction of fibrinogen with monocytes was studied. After stimulation with ADP (10 microM) or thrombin (1 U/ml), platelet-free suspensions of human monocytes bind 125I-fibrinogen with two different affinities in a specific and Ca2+-dependent reaction with saturation at 5.80-7.35 X 10(-7) M of added protein. The binding of fibrinogen to specific receptors on monocytes induces the procoagulant activity of these cells. Thrombasthenic cells or normal monocytes preincubated with a monoclonal antibody to the platelet glycoprotein IIb/IIIa complex (10E5) do not bind fibrinogen and have no procoagulant activity. Metabolic studies with [35S]methionine revealed that cultured monocytes actually synthesize a surface antigen precipitated by 10E5 antibody as a major band with 92,000 relative molecular weight. Our data indicate that monocytes express receptors for fibrinogen only in part related to the platelet glycoprotein IIb/IIIa complex. Furthermore, the binding of fibrinogen to monocytes enhances the cooperation of these cells in hemostasis.

References

Mar 1, 1977·The Journal of Clinical Investigation·H RothbergerJ H Vaughan
Nov 1, 1979·The Journal of Clinical Investigation·J S Bennett, G Vilaire
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Jan 1, 1977·The Journal of Experimental Medicine·L A Sherman, J Lee
Jul 1, 1975·British Journal of Haematology·R P RiversW L Weston
May 1, 1985·The Journal of Experimental Medicine·K J ClemetsonM Baggiolini
Feb 1, 1972·British Journal of Haematology·J F MustardM A Packham
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Z M RuggeriL de Marco
Aug 1, 1983·The Journal of Experimental Medicine·N A PawlowskiW A Scott
Aug 1, 1981·The Journal of Clinical Investigation·L De Marco, S S Shapiro
Feb 1, 1983·The Journal of Experimental Medicine·N Hogg
Apr 1, 1983·Analytical Biochemistry·G P TuszynskiS Srivastava
Sep 1, 1982·The Journal of Clinical Investigation·G J Broze
Mar 1, 1980·The Journal of Experimental Medicine·K AlitaloA Vaheri
May 1, 1961·Archives of Biochemistry and Biophysics·J MOLNAR, L LORAND
Aug 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·L A KAZALL M TOCANTINS

❮ Previous
Next ❯

Citations

Feb 24, 1988·Biochimica Et Biophysica Acta·K J Clemetson, E F Lüscher
Dec 9, 2003·Research in Veterinary Science·A L OuelletteM F Heath
Aug 23, 2002·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Andrea GessnerRainer Müller
Apr 16, 1998·British Journal of Pharmacology·J JinS P Kunapuli
Mar 23, 2000·Respirology : Official Journal of the Asian Pacific Society of Respirology·S Z Wang, K D Forsyth
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S D WrightJ D Loike
Nov 1, 1986·The Journal of Experimental Medicine·D C Altieri, P M Mannucci
May 23, 2001·The Journal of Experimental Medicine·C B ForsythE F Plow
Oct 18, 2001·Current Opinion in Hematology·B A Bouchard, P B Tracy
Aug 6, 2002·Journal of Biomaterials Science. Polymer Edition·Mingchao ShenThomas A Horbett
Jul 20, 2006·Blood·Valeryi K LishkoTatiana Ugarova
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·C RogersD I Simon
May 1, 1992·European Journal of Epidemiology·G Di MinnoM Mancini
Sep 11, 2007·Journal of Immunological Methods·Peter R HoffmannMarla J Berry
Nov 1, 1988·Thrombosis Research·J H MorrisseyT S Edgington
Jan 1, 1990·Thrombosis Research. Supplement·G Di MinnoE Tremoli
Jan 1, 1989·Critical Reviews in Oncology/hematology·K L Kaplan, A Bini
Jan 28, 2014·Biomaterials·Toral D ZaveriBenjamin G Keselowsky
Aug 8, 2015·BioMed Research International·Susan N ChristoJohn D Hayball
Apr 11, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Akiko OkadaKoichi Orino
Jan 15, 1989·Thrombosis Research·E Grau, L A Moroz
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·A BiniK L Kaplan
Mar 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·K S SakariassenH R Baumgartner
Jan 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·G Di Minno, M Mancini
Jun 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·E StragliottoE Tremoli

❮ Previous
Next ❯

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